Ríhová B, Ríha I
Am J Reprod Immunol (1980). 1981 Aug;1(4):168-73. doi: 10.1111/j.1600-0897.1981.tb00030.x.
The analysis of anti-sheep red blood cell (SRBC) antibody production in the congenic resistant (CR) mouse strains A/J and A.BY, B10 and B10.A showed that the level of IgG antibodies after immunization with SRBC is controlled by a gene(s) localized in the H-2 complex. The use of h2,h4, and i5H-2 recombinant haplotypes allowed us to map this gene into a region proximally defined by subregion I-J and distally by region H-2G. The IgG antibody level is simultaneously under the influence of non-H-2 genes, of which those of the A/J strain origin determine the high IgG level and those of the B10 strain origin determine the low IgG level. Weights of spleens of the A/J and B10 mice before and after primary and secondary immunization with SRBC were compared. Before immunization, spleens of the A/J mice were approximately 10% lighter than those of the B10 mice (0.08 g vs 0.09 g). After the first immunization the spleen weights equalized (0=0.12 g). Four days after the second immunization the weight of the spleens of A/J mice increased by 312% (relative to the nonimmune state) while that of the B10 mice increased by only 74%. These findings indicate that the cell antigen-specific proliferation in spleens of A/J mice was considerably higher than that in spleens of B10 mice.
对同源抗性（CR）小鼠品系A/J和A.BY、B10和B10.A中抗绵羊红细胞（SRBC）抗体产生情况的分析表明，用SRBC免疫后IgG抗体水平受位于H-2复合体中的一个或多个基因控制。利用h2、h4和i5H-2重组单倍型，我们能够将该基因定位到一个区域，该区域近端由I-J亚区界定，远端由H-2G区域界定。IgG抗体水平同时受到非H-2基因的影响，其中源自A/J品系的基因决定了高IgG水平，而源自B10品系的基因决定了低IgG水平。比较了A/J和B10小鼠在用SRBC进行初次和二次免疫前后脾脏的重量。免疫前，A/J小鼠的脾脏比B10小鼠的脾脏轻约10%（0.08 g对0.09 g）。初次免疫后脾脏重量相等（均为0.12 g）。二次免疫后四天，A/J小鼠脾脏重量相对于非免疫状态增加了312%，而B10小鼠脾脏重量仅增加了74%。这些发现表明，A/J小鼠脾脏中细胞抗原特异性增殖明显高于B10小鼠脾脏中的增殖。
