Gonzalez I L, Sheppard D E
J Bacteriol. 1977 May;130(2):684-91. doi: 10.1128/jb.130.2.684-691.1977.
Partial reversion mutants derived from a strain containing a strongly polar initiator-defective mutation (araI1036) in the L-arabinose operon were found to have several characteristics expected of mutants with reduced initiator function. These reversion mutations are cotransduced with the ara region and are probably within the araI region. Furthermore, they permit induction of the L-arabinose operon to a level only one-third of the normal wild-type level. These partially functional initiator regions reduce the expression of structural genes in the cis position only; they function quite independently of wild-type or defective initiator regions in the trans position. These mutants exhibit a two- to threefold increase in the rate of expression of ara operon genes within one-tenth of a generation after a shift of the growth temperature from 28 to 42 degrees C. This suggests that the temperature optimum for initiation of operon expression is higher for the partial revertant strains than it is for strains containing a wild-type initiator region.
从一株在L -阿拉伯糖操纵子中含有强极性起始子缺陷突变（araI1036）的菌株衍生而来的部分回复突变体，被发现具有一些预期的起始子功能降低的突变体特征。这些回复突变与ara区域共转导，并且可能位于araI区域内。此外，它们使L -阿拉伯糖操纵子的诱导水平仅为正常野生型水平的三分之一。这些部分功能的起始子区域仅降低顺式位置结构基因的表达；它们在反式位置上与野生型或缺陷型起始子区域完全独立发挥作用。这些突变体在生长温度从28℃转变到42℃后的十分之一代内，ara操纵子基因的表达速率增加了两到三倍。这表明部分回复菌株中操纵子表达起始的最适温度高于含有野生型起始子区域的菌株。
