Wilcox G, Boulter J, Lee N
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3635-9. doi: 10.1073/pnas.71.9.3635.
The protein product of the regulatory gene araC can be synthesized in a cell-free, protein-synthesizing system programmed with a lambdaparaC(+)B DNA template. Hybrid, renatured phage DNA molecules prepared with DNA from phages lambdaparaC(+)B and lambdaparaC3B (araC3 is a nonsense mutation) were used to program the cell-free synthesis of the araC protein. The findings observed lead to the conclusion that the codogenic strand of the araC gene is on the light strand of the phage DNA. The araB gene is on the heavy strand, as determined by DNA.RNA hybridization. Thus, with regard to the standard E. coli map, araC is transcribed in a clockwise direction, whereas transcription of the araBAD operon has a counterclockwise orientation. The technique described should allow one to determine the direction of transcription of any gene that can be incorporated into the genome of a specialized transducing phage.
调节基因araC的蛋白质产物可以在以λaraC(+)B DNA模板编程的无细胞蛋白质合成系统中合成。用来自噬菌体λaraC(+)B和λaraC3B（araC3是一个无义突变）的DNA制备的杂交、复性噬菌体DNA分子被用于编程无细胞合成araC蛋白。观察到的结果得出结论：araC基因的编码链位于噬菌体DNA的轻链上。如通过DNA-RNA杂交所确定的，araB基因位于重链上。因此，就标准大肠杆菌图谱而言，araC是按顺时针方向转录的，而araBAD操纵子的转录具有逆时针方向。所描述的技术应该能使人们确定任何可以整合到特异性转导噬菌体基因组中的基因的转录方向。
