Daugherty Alan, Milewicz Dianna M, Dichek David A, Ghaghada Ketan B, Humphrey Jay D, LeMaire Scott A, Li Yanming, Mallat Ziad, Saeys Yvan, Sawada Hisashi, Shen Ying H, Suzuki Toru, Zhou Zhen
Department of Physiology, Saha Cardiovascular Research Center, Saha Aortic Center, University of Kentucky (A.D., H.S.).
Division of Medical Genetics, Department of Internal Medicine, McGovern Medical School, The University of Texas Health Science Center at Houston (D.M.M., Z.Z.).
Arterioscler Thromb Vasc Biol. 2025 May;45(5):609-631. doi: 10.1161/ATVBAHA.124.320259. Epub 2025 Mar 13.
There is a recent dramatic increase in research on thoracic aortic diseases that includes aneurysms, dissections, and rupture. Experimental studies predominantly use mice in which aortopathy is induced by chemical interventions, genetic manipulations, or both. Many parameters should be deliberated in experimental design in concert with multiple considerations when providing dimensional data and characterization of aortic tissues. The purpose of this review is to provide recommendations on guidance in (1) the selection of a mouse model and experimental conditions for the study, (2) parameters for standardizing detection and measurements of aortic diseases, (3) meaningful interpretation of characteristics of diseased aortic tissue, and (4) reporting standards that include rigor and transparency.
最近，关于胸主动脉疾病（包括动脉瘤、夹层和破裂）的研究急剧增加。实验研究主要使用小鼠，通过化学干预、基因操作或两者结合来诱导主动脉病变。在提供主动脉组织的尺寸数据和特征描述时，实验设计中应结合多种考虑因素，仔细斟酌许多参数。本综述的目的是就以下方面提供指导建议：（1）研究中小鼠模型和实验条件的选择；（2）主动脉疾病检测和测量标准化的参数；（3）对病变主动脉组织特征的有意义解释；（4）包括严谨性和透明度的报告标准。
