Ebrahimvand Zahra, Daliri Mohammad Reza
Neuroscience & Neuroengineering Research Lab., Biomedical Engineering Department, School of Electrical Engineering, Iran University of Science & Technology, Tehran, Iran.
Brain Behav. 2025 Mar;15(3):e70360. doi: 10.1002/brb3.70360.
Oriented grating is usually employed in visual science experiments as a prominent property of neurons in the visual cortices. Previous studies have shown that the study of mouse vision can make a significant contribution to the field of neuroscience research, and also the local field potential (LFP) analysis could contain more information and give us a better view of brain function.
In this research, cross-frequency coupling is employed to assess the grating orientation perception in V1 and lateromedial (LM) of 10 mice. The experimental data were collected using chronically implanted multielectrode arrays, involving area V1 recording of five mice and area LM recording of five mice separately, performing a passive visual task. Two criteria known as phase-amplitude coupling (PAC) and amplitude-amplitude coupling (AAC) were exploited to analyze the characteristics of cross-frequency coupling of LFP signals in the experiment consisting of first-order and second-order drifting sinusoidal grating stimuli with different orientations.
It was found that in area LM the correlation between phase of lower than 8 Hz band signal and amplitude of above 100 Hz band signal can be significantly different for orientations and stimulus conditions simultaneously. In area V1, this difference was observed in amplitude correlation between 12 and 30 Hz and more than 70 Hz subbands.
In conclusion, PAC and AAC can be proper features in orientation perception detection. Our results suggest that in both areas, the significant role of high-band and low-band oscillations of LFPs discloses the reliability of these bands and generally LFP signals in mice visual perception.
定向光栅通常用于视觉科学实验，因为它是视觉皮层神经元的一个显著特性。先前的研究表明，对小鼠视觉的研究可以为神经科学研究领域做出重大贡献，而且局部场电位（LFP）分析可能包含更多信息，能让我们更好地了解大脑功能。
在本研究中，采用交叉频率耦合来评估10只小鼠初级视皮层（V1）和外侧内皮层（LM）的光栅方向感知。使用长期植入的多电极阵列收集实验数据，分别对5只小鼠的V1区域和5只小鼠的LM区域进行记录，执行被动视觉任务。在由具有不同方向的一阶和二阶漂移正弦光栅刺激组成的实验中，利用称为相位-幅度耦合（PAC）和幅度-幅度耦合（AAC）的两个标准来分析LFP信号的交叉频率耦合特征。
发现在LM区域，低于8Hz频段信号的相位与高于100Hz频段信号的幅度之间的相关性在方向和刺激条件上同时存在显著差异。在V1区域，在12至30Hz和高于70Hz子频段的幅度相关性中观察到这种差异。
总之，PAC和AAC可以作为方向感知检测的合适特征。我们的结果表明，在这两个区域，LFP的高频和低频振荡的重要作用揭示了这些频段以及一般LFP信号在小鼠视觉感知中的可靠性。
