Xu Zhonglei, Zhu Qiyin, Yang Ying, Shan Wenjun, Jiang Fang, Zou Qianli, Wang Wenyan
The Second Affiliated Hospital of Anhui Medical University, Hefei, 230601, People's Republic of China.
School of Pharmacy, Anhui Medical University, Hefei, 230032, People's Republic of China.
J Mol Histol. 2025 Mar 14;56(2):107. doi: 10.1007/s10735-025-10383-w.
Pelvic organ prolapse (POP) is a prevalent condition that affects postmenopausal women and significantly impacts their quality of life. The most common potential causes include vaginal delivery, age, and obesity. However, the specific pathophysiological mechanisms involved remain unclear. Therefore, we conducted a comparative analysis of vaginal anterior wall-associated proteins between POP patients and non-POP patients using Masson staining, immunohistochemistry, western blotting, and real-time quantitative fluorescence PCR. Additionally, we investigated the effect of ciliary neurotrophic factor (CNTF) on collagen secretion by fibroblasts in cell culture and instantaneous transfection experiments. Furthermore, the role of CNTF in the development of POP was investigated by constructing a rat prolapse model in which bilateral ovaries were removed and vaginal delivery was simulated. The findings indicated that the anterior vaginal wall of POP patients exhibited high CNTF expression, low collagen I expression, and high collagen III expression. Furthermore, cell transfection experiments demonstrated that CNTF may inhibit collagen I expression and promote collagen III expression by activating the JAK2-STAT3 pathway. A rat model constructed by simulating vaginal delivery after bilateral ovary removal is also an appropriate animal model for studying POP.
盆腔器官脱垂（POP）是一种常见病症，影响绝经后女性，并对其生活质量产生重大影响。最常见的潜在病因包括阴道分娩、年龄和肥胖。然而，其中具体的病理生理机制仍不清楚。因此，我们使用Masson染色、免疫组织化学、蛋白质印迹法和实时定量荧光PCR对POP患者和非POP患者的阴道前壁相关蛋白进行了比较分析。此外，我们在细胞培养和瞬时转染实验中研究了睫状神经营养因子（CNTF）对成纤维细胞胶原蛋白分泌的影响。此外，通过构建双侧卵巢切除并模拟阴道分娩的大鼠脱垂模型，研究了CNTF在POP发生发展中的作用。研究结果表明，POP患者的阴道前壁表现出CNTF高表达、I型胶原蛋白低表达和III型胶原蛋白高表达。此外，细胞转染实验表明，CNTF可能通过激活JAK2-STAT3通路抑制I型胶原蛋白表达并促进III型胶原蛋白表达。双侧卵巢切除后模拟阴道分娩构建的大鼠模型也是研究POP的合适动物模型。
