Vafaeva Olga, Namchaiw Poommaree, Murray Karl, Diaz Elva, Cheng Hwai-Jong
Center for Neuroscience, University of California, Davis, Davis, CA 95616, USA; Department of Pharmacology, School of Medicine, University of California, Davis, Davis, CA 95616, USA.
Center for Neuroscience, University of California, Davis, Davis, CA 95616, USA; Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.
STAR Protoc. 2025 Mar 21;6(1):103692. doi: 10.1016/j.xpro.2025.103692. Epub 2025 Mar 13.
The neurosphere assay is the gold standard for assessing the proliferative and differentiation capacities of neural progenitor cells (NPCs). Here, we present a protocol for isolating, propagating, and maintaining hippocampal neurospheres from adult and aged mice and differentiating cultured NPCs into neurons and astrocytes. We describe steps for establishing a heterochronic co-culture of neurosphere-derived cells with primary neurons. Using neurospheres from old animals enables investigation of the effects of aging on the development and differentiation of newborn neurons.
神经球分析是评估神经祖细胞（NPCs）增殖和分化能力的金标准。在此，我们提供了一种从成年和老年小鼠中分离、扩增和维持海马神经球，并将培养的NPCs分化为神经元和星形胶质细胞的方案。我们描述了建立神经球来源细胞与原代神经元异时共培养的步骤。使用来自老年动物的神经球能够研究衰老对新生神经元发育和分化的影响。
