Björkman S
J Chromatogr. 1985 May 3;339(2):339-46. doi: 10.1016/s0378-4347(00)84661-7.
A rapid method for the determination of (+)- and (-)-indoprofen in blood plasma has been developed and applied to pharmacokinetic work. By means of ethyl chloroformate, indoprofen is coupled to leucinamide, a reaction which is complete in less than 3 min at room temperature. The diastereoisomeric derivatives are then separated by reversed-phase high-performance liquid chromatography. In two surgical patients given racemic indoprofen intravenously, the pharmacologically active (+)-enantiomer had a lower clearance and a lower distribution volume than the (-)-enantiomer, which gave it a slightly longer half-life.
已开发出一种快速测定血浆中（+）-和（-）-吲哚洛芬的方法，并将其应用于药代动力学研究。通过氯甲酸乙酯，吲哚洛芬与亮氨酰胺偶联，该反应在室温下不到3分钟即可完成。然后通过反相高效液相色谱法分离非对映异构体衍生物。在两名接受外消旋吲哚洛芬静脉注射的外科手术患者中，药理活性（+）-对映体的清除率和分布体积低于（-）-对映体，这使其半衰期略长。
