Yong D C, Thompson J S, Prytula A
J Clin Microbiol. 1985 Jun;21(6):914-8. doi: 10.1128/jcm.21.6.914-918.1985.
A method for rapid screening of isolates of pathogenic members of the family Enterobacteriaceae is described. Flow charts are used in conjunction with triple sugar iron agar, o-nitrophenyl-beta-D-galactopyranoside-phenylalanine-motility sulfate screening media, oxidase test, and six rapid biochemical tests, namely, lysine decarboxylase, urease, indole, esculin hydrolysis, malonate, and xylose. This scheme is used to provide an inexpensive but rapid presumptive identification of Salmonella, Shigella, Edwardsiella, Aeromonas, Plesiomonas, Vibrio, and Yersinia isolates from stool cultures.
本文描述了一种快速筛查肠杆菌科致病成员分离株的方法。流程图与三糖铁琼脂、邻硝基苯基-β-D-吡喃半乳糖苷-苯丙氨酸-动力-硫酸盐筛选培养基、氧化酶试验以及六项快速生化试验(即赖氨酸脱羧酶、脲酶、吲哚、七叶苷水解、丙二酸盐和木糖试验)联合使用。该方案用于从粪便培养物中对沙门氏菌、志贺氏菌、爱德华氏菌、气单胞菌、邻单胞菌、弧菌和耶尔森氏菌分离株进行廉价但快速的初步鉴定。