厌氧菌的培养与鉴定：一种适用于常规临床实验室的系统。
Recovery and identification of anaerobes: a system suitable for the routine clinical laboratory.
作者信息
Ellner P D, Granato P A, May C B
出版信息
Appl Microbiol. 1973 Dec;26(6):904-13. doi: 10.1128/am.26.6.904-913.1973.
Abstract
A system for the isolation of anaerobes based upon the use of reducible solid media is described. Plates of reducible media prepared and stored aerobically were reduced before use by incubation in a GasPak jar for 24 h. Clinical specimens for culture were carefully selected. The value of Amies transport medium was confirmed. Selective and nonselective formulations of reducible media were used for primary isolation. Abbreviated identification schemes based in part on gas-liquid chromatography are presented. The suitability of this system for improving the recovery and identification of anaerobes in a routine clinical laboratory is documented.
摘要
本文描述了一种基于使用可还原固体培养基分离厌氧菌的系统。需氧条件下制备和储存的可还原培养基平板，在使用前通过在厌氧罐中培养24小时进行还原。仔细挑选用于培养的临床标本。证实了阿氏运送培养基的价值。可还原培养基的选择性和非选择性配方用于初次分离。提出了部分基于气-液相色谱的简化鉴定方案。记录了该系统在常规临床实验室中用于提高厌氧菌回收率和鉴定的适用性。
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