对粪便标本中分离出的沙门氏菌、志贺氏菌、耶尔森氏菌和气单胞菌进行快速生化筛查。
Rapid biochemical screening for Salmonella, Shigella, Yersinia, and Aeromonas isolates from stool specimens.
作者信息
De Ryck R, Struelens M J, Serruys E
机构信息
Laboratoire de Microbiologie, Hôpital Erasme, Université Libre de Bruxelles, Belgium.
出版信息
J Clin Microbiol. 1994 Jun;32(6):1583-5. doi: 10.1128/jcm.32.6.1583-1585.1994.
Four screens for the rapid (4 to 6 h) biochemical detection of pathogens from enteric isolation media are described. The Salmonella screen consisted of Kligler iron agar (KIA), motility-indole-urea-tryptophan-deamination semisolid medium (MIU-TDA), and the o-nitrophenyl-beta-D-galactopyranoside (ONPG) test; the Shigella screen consisted of KIA, MIU-TDA, the ONPG test, and the lysine decarboxylation-indole test; the Yersinia screen consisted of a rhamnose broth; the Aeromonas screen consisted of a xylose agar plate. When tested on 2,102 fresh isolates and 71 stock strains, the screens correctly detected 212 enteric pathogens (sensitivity, 100%), with a specificity of 98.1%.
本文描述了四种用于从肠道分离培养基中快速（4至6小时）进行病原体生化检测的筛选方法。沙门氏菌筛选方法包括克氏铁琼脂（KIA）、动力-吲哚-尿素-色氨酸-脱氨基半固体培养基（MIU-TDA）和邻硝基苯-β-D-吡喃半乳糖苷（ONPG）试验；志贺氏菌筛选方法包括KIA、MIU-TDA、ONPG试验和赖氨酸脱羧酶-吲哚试验；耶尔森氏菌筛选方法包括鼠李糖肉汤；气单胞菌筛选方法包括木糖琼脂平板。在对2102株新鲜分离株和71株标准菌株进行测试时，这些筛选方法正确检测出212种肠道病原体（灵敏度为100%），特异性为98.1%。
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