小麦叶枯病抗性基因Stb15编码一种凝集素受体样激酶。

Septoria tritici blotch resistance gene Stb15 encodes a lectin receptor-like kinase.

作者信息

Hafeez Amber N, Chartrain Laetitia, Feng Cong, Cambon Florence, Clarke Martha, Griffiths Simon, Hayta Sadiye, Jiang Mei, Keller Beat, Kirby Rachel, Kolodziej Markus C, Powell Oliver R, Smedley Mark A, Steuernagel Burkhard, Xian Wenfei, Wingen Luzie U, Cheng Shifeng, Saintenac Cyrille, Wulff Brande B H, Brown James K M

机构信息

John Innes Centre, Norwich Research Park, Norwich, UK.

Plant Science Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.

出版信息

Nat Plants. 2025 Mar;11(3):410-420. doi: 10.1038/s41477-025-01920-2. Epub 2025 Mar 14.

DOI:10.1038/s41477-025-01920-2

PMID:40087541

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11928318/

Abstract

Septoria tritici blotch (STB), caused by the Dothideomycete fungus Zymoseptoria tritici, is one of the most damaging diseases of bread wheat (Triticum aestivum) and the target of costly fungicide applications. In line with the fungus's apoplastic lifestyle, STB resistance genes isolated to date encode receptor-like kinases (RLKs) including a wall-associated kinase (Stb6) and a cysteine-rich kinase (Stb16q). Here we used genome-wide association studies on a diverse panel of 300 whole-genome shotgun-sequenced wheat landraces (WatSeq consortium) to identify a 99-kb region containing six candidates for the Stb15 resistance gene. Mutagenesis and transgenesis confirmed a gene encoding an intronless G-type lectin RLK as Stb15. The characterization of Stb15 exemplifies the unexpected diversity of RLKs conferring Z. tritici resistance in wheat.

摘要

小麦黄斑叶枯病（STB）由座囊菌纲真菌小麦壳针孢引起，是面包小麦（普通小麦）最具破坏性的病害之一，也是昂贵杀菌剂应用的目标对象。与该真菌的质外体生活方式一致，迄今分离出的STB抗性基因编码类受体激酶（RLK），包括一个壁相关激酶（Stb6）和一个富含半胱氨酸的激酶（Stb16q）。在这里，我们对300个全基因组鸟枪法测序的小麦地方品种（WatSeq联盟）组成的多样化群体进行全基因组关联研究，以鉴定一个包含Stb15抗性基因六个候选基因的99千碱基区域。诱变和转基因证实一个编码无内含子G型凝集素RLK的基因为Stb15。Stb15的特性体现了赋予小麦对小麦壳针孢抗性的RLK出人意料的多样性。