Hiserodt J C, Laybourn K A, Varani J
J Immunol. 1985 Aug;135(2):1484-7.
We have identified a structure on the surface of murine NK cells that is immunochemically cross-reactive with laminin. Treatment of normal CBA/J spleen cells with monospecific anti-laminin serum plus complement completely eliminated NK cytolytic activity against YAC-1 or RL male 1 target cells. In the absence of added complement, spleen cells preincubated with anti-laminin serum were also reduced in their cytolytic activity due to a reduced capacity to bind to the target cells. Treatment with anti-asialo GM1 serum plus complement also eliminated NK activity, but pretreatment of NK cells with anti-asialo GM1 in the absence of complement did not reduce cytolytic activity. Thus, anti-laminin and anti-asialo GM1 bind to structures on the surface of NK cells that distinguish functional (laminin) from nonfunctional (asialo GM1) sites. Flow cytometric analysis revealed that approximately 15% of normal nonadherent splenic lymphocytes expressed laminin-like structures, whereas 16% expressed asialo GM1 and 19% expressed the NK alloantigen NK 2.1. Treatment of alloimmune cytotoxic T lymphocytes (CTL) with anti-laminin plus complement did not affect CTL activity. Thus, anti-laminin serum appears to detect a cell surface structure present on the NK subset of lymphocytes.
我们在小鼠自然杀伤（NK）细胞表面鉴定出一种结构，该结构与层粘连蛋白存在免疫化学交叉反应。用单特异性抗层粘连蛋白血清加补体处理正常CBA/J脾细胞，可完全消除其对YAC-1或RL雄性1靶细胞的NK细胞溶解活性。在不添加补体的情况下，预先用抗层粘连蛋白血清孵育的脾细胞，由于其与靶细胞结合能力降低，其细胞溶解活性也降低。用抗去唾液酸GM1血清加补体处理也可消除NK活性，但在无补体情况下用抗去唾液酸GM1预处理NK细胞并不会降低细胞溶解活性。因此，抗层粘连蛋白和抗去唾液酸GM1与NK细胞表面的结构结合，这些结构区分了功能性位点（层粘连蛋白）和非功能性位点（去唾液酸GM1）。流式细胞术分析显示，约15%的正常非贴壁脾淋巴细胞表达层粘连蛋白样结构，而16%表达去唾液酸GM1，19%表达NK同种异体抗原NK 2.1。用抗层粘连蛋白加补体处理同种异体免疫细胞毒性T淋巴细胞（CTL）不会影响CTL活性。因此，抗层粘连蛋白血清似乎检测到淋巴细胞NK亚群上存在的一种细胞表面结构。
