Kasai M, Iwamori M, Nagai Y, Okumura K, Tada T
Eur J Immunol. 1980 Mar;10(3):175-80. doi: 10.1002/eji.1830100304.
Cytotoxic treatment with rabbit antiserum raised against purified glycosphingolipid "asialo GM1" was capable of eliminating natural killer (NK) activity of spleen cells from different inbred mouse strains including CBA/J, C57BL/6, BALB/c, AKR, and athymic nude mice. The anti-asialo GM1 antiserum showed little cross-reactivity with structurally related glycolipids, e.g. GM), GD 1 b and asialo GM2 in the microflocculation test. The specific reactivity of this antiserum with NK cells was confirmed by the quantitative absorption of anti-NK activity with graded amounts of asialo GM1 but not with other glycosphingolipids. The absorption of anti-brain-associated T cell antigen (anti-BAT) with asialo GM1 also effectively diminished its anti-NK activity, leaving the ability to kill T cells intact. This suggests that the antibody to asialo GM1 is responsible for the anti-NK activity contained in the anti-BAT antiserum. In contrast to the extreme sensitivity of NK cells to anti-asialo GM1, alloreactive cytotoxic T killer cells generated in the mixed lymphocyte culture were not killed by anti-asialo GM1 and complement. These results indicate that asialo GM1 is expressed on mouse NK cells in a high concentration.
用针对纯化糖鞘脂“去唾液酸GM1”制备的兔抗血清进行细胞毒性处理，能够消除来自不同近交系小鼠（包括CBA/J、C57BL/6、BALB/c、AKR和无胸腺裸鼠）脾细胞的自然杀伤（NK）活性。在微絮凝试验中，抗去唾液酸GM1抗血清与结构相关的糖脂（如GM1、GD1b和去唾液酸GM2）几乎没有交叉反应。通过用不同量的去唾液酸GM1而非其他糖鞘脂对抗NK活性进行定量吸收，证实了该抗血清与NK细胞的特异性反应性。用去唾液酸GM1吸收抗脑相关T细胞抗原（抗BAT）也有效降低了其抗NK活性，同时保留了其杀伤T细胞的能力。这表明去唾液酸GM1抗体是抗BAT抗血清中所含抗NK活性的原因。与NK细胞对抗去唾液酸GM1的极端敏感性相反，混合淋巴细胞培养中产生的同种异体反应性细胞毒性T杀伤细胞不会被抗去唾液酸GM1和补体杀死。这些结果表明，去唾液酸GM1在小鼠NK细胞上高浓度表达。
