Bhakdi S, Jenne D, Hugo F
J Immunol Methods. 1985 Jun 12;80(1):25-32. doi: 10.1016/0022-1759(85)90160-7.
Immunoprecipitates formed in conventional electroimmunoassays were solubilized by brief immersion of the agarose gels in sodium dodecylsulfate. The proteins were then transferred to nitrocellulose sheets by electroblotting. The blotted proteins were readily amenable to analyses by non-precipitating monoclonal antibodies and the immunoblots were developed with second antibody/biotin-streptavidin-peroxidase staining. The electroimmunoassay-immunoblot (EIA-IB) method is of value in (1) specificity assays of monoclonal antibodies in crossed immunoelectrophoresis; (2) analysis of specific molecular interactions between proteins; (3) rapid screening and simple identification of monoclonal antibodies by line immunoelectrophoresis-immunoblotting.
在传统的电免疫分析中形成的免疫沉淀物，通过将琼脂糖凝胶短暂浸泡在十二烷基硫酸钠中而溶解。然后通过电印迹法将蛋白质转移到硝酸纤维素膜上。印迹的蛋白质很容易用非沉淀性单克隆抗体进行分析，免疫印迹用二抗/生物素-链霉亲和素-过氧化物酶染色法显影。电免疫分析-免疫印迹（EIA-IB）方法在以下方面具有价值：（1）交叉免疫电泳中单抗的特异性分析；（2）蛋白质间特异性分子相互作用的分析；（3）通过线免疫电泳-免疫印迹法对单克隆抗体进行快速筛选和简单鉴定。
