Ekwall K, Söderholm J, Wadström T
J Immunol Methods. 1976;12(1-2):103-15. doi: 10.1016/0022-1759(76)90100-9.
A technique, disc-crossed immunoelectrophoresis, is described to provide a simple and convenient means of performing electrophoresis in polyacrylamide gel in a discontinuous buffer system (disc electrophoresis) followed by electrophoresis into antibody-containing agarose gel. A simple washing of the polyacrylamide gel in distilled water combined with a "laying-on" method, seems to be a satisfactory and rapid way to obtain immunoprecipitation patterns of high quality using commercially available agarose in the second electrophoresis.
本文描述了一种盘状交叉免疫电泳技术，该技术提供了一种简单便捷的方法，可在不连续缓冲系统（盘状电泳）的聚丙烯酰胺凝胶中进行电泳，随后再在含抗体的琼脂糖凝胶中进行电泳。在第二次电泳中，将聚丙烯酰胺凝胶在蒸馏水中简单冲洗，并结合“铺胶”方法，似乎是使用市售琼脂糖获得高质量免疫沉淀图谱的一种令人满意且快速的方法。
