Rega Camilla, Tsitsa Ifigenia, Roumeliotis Theodoros I, Krystkowiak Izabella, Portillo Maria, Yu Lu, Vorhauser Julia, Pines Jonathon, Mansfeld Jörg, Choudhary Jyoti, Davey Norman E
Division of Cancer Biology, The Institute of Cancer Research, London, UK.
Nat Commun. 2025 Mar 16;16(1):2579. doi: 10.1038/s41467-025-57537-8.
The cell cycle governs a precise series of molecular events, regulated by coordinated changes in protein and phosphorylation abundance, that culminates in the generation of two daughter cells. Here, we present a proteomic and phosphoproteomic analysis of the human cell cycle in hTERT-RPE-1 cells using deep quantitative mass spectrometry by isobaric labelling. By analysing non-transformed cells and improving the temporal resolution and coverage of key cell cycle regulators, we present a dataset of cell cycle-dependent protein and phosphorylation site oscillation that offers a foundational reference for investigating cell cycle regulation. These data reveal regulatory intricacies including proteins and phosphorylation sites exhibiting cell cycle-dependent oscillation, and proteins targeted for degradation during mitotic exit. Integrated with complementary resources, our data link cycle-dependent abundance dynamics to functional changes and are accessible through the Cell Cycle database (CCdb), an interactive web-based resource for the cell cycle community.
细胞周期控制着一系列精确的分子事件，这些事件由蛋白质和磷酸化丰度的协调变化所调节，最终导致两个子细胞的产生。在此，我们使用等压标记的深度定量质谱技术，对hTERT-RPE-1细胞中的人类细胞周期进行了蛋白质组学和磷酸蛋白质组学分析。通过分析未转化细胞并提高关键细胞周期调节因子的时间分辨率和覆盖范围，我们呈现了一个细胞周期依赖性蛋白质和磷酸化位点振荡的数据集，为研究细胞周期调控提供了基础参考。这些数据揭示了调控的复杂性，包括表现出细胞周期依赖性振荡的蛋白质和磷酸化位点，以及在有丝分裂退出期间被靶向降解的蛋白质。与互补资源相结合，我们的数据将周期依赖性丰度动态与功能变化联系起来，并且可以通过细胞周期数据库（CCdb）获取，CCdb是一个基于网络的交互式资源，供细胞周期研究领域的人员使用。
