Zhu Liwei, Song Guangjie, Zhang Wentian, Wu Yifan, Chen Yuling, Song Jiayi, Wang Deliang, Li Guoxin, Tang Ben Zhong, Li Ying
Innovation Research Center for AIE Pharmaceutical Biology, Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, the NMPA and State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences and the Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 511436, China.
Center for AIE Research, Shenzhen Key Laboratory of Polymer Science and Technology, Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen, 518060, China.
Nat Commun. 2025 Mar 16;16(1):2578. doi: 10.1038/s41467-025-57533-y.
Multimodal phototheranostics utilizing single molecules offer a "one-and-done" approach, presenting a convenient and effective strategy for cancer therapy. However, therapies based on conventional photosensitizers often suffer from limitations such as a single photosensitizing mechanism, restricted tumor penetration and retention, and the requirement for multiple irradiations, which significantly constrain their application. In this report, we present an aggregation-induced emission luminogen (AIEgen) bacteria hybrid bionic robot to address above issues. This bionic robot is composed of multifunctional AIEgen (INX-2) and Escherichia coli Nissle 1917 (EcN), i.e., EcN@INX-2. The EcN@INX-2 bionic robot exhibits near-infrared II (NIR-II) fluorescence emission and demonstrates efficient photodynamic and photothermal effects, as well as tumor-targeting capabilities. These features are facilitated by the complementary roles of INX-2 and EcN. The robot successfully enables in vivo multimodal imaging and therapy of colon cancer models in female mice through various mechanisms, including the activation of anti-tumor immunity, as well as photodynamic and photothermal therapy. Our study paves an avenue for designing multifunctional diagnostic agents for targeted colon cancer therapy through image-guided combinational immunotherapy.
利用单分子的多模态光诊疗技术提供了一种“一次性完成”的方法，为癌症治疗提供了一种便捷有效的策略。然而，基于传统光敏剂的疗法常常存在诸如单一光敏机制、肿瘤穿透和滞留受限以及需要多次照射等局限性，这显著限制了它们的应用。在本报告中，我们提出了一种聚集诱导发光 luminogen（AIEgen）细菌杂交仿生机器人来解决上述问题。这种仿生机器人由多功能 AIEgen（INX-2）和大肠杆菌 Nissle 1917（EcN）组成，即 EcN@INX-2。EcN@INX-2 仿生机器人表现出近红外 II（NIR-II）荧光发射，并展示出高效的光动力和光热效应以及肿瘤靶向能力。这些特性得益于 INX-2 和 EcN 的互补作用。该机器人通过多种机制成功实现了雌性小鼠结肠癌模型的体内多模态成像和治疗，包括激活抗肿瘤免疫以及光动力和光热疗法。我们的研究为通过图像引导的联合免疫疗法设计用于靶向结肠癌治疗的多功能诊断剂铺平了道路。
