Sandquist D, Williams T H, Sahu S K, Kataoka S
Department of Anatomy, College of Medicine, University of Iowa, Iowa City 52242.
Exp Cell Res. 1978 May;113(2):375-81. doi: 10.1016/0014-4827(78)90378-6.
Dexamethasone induces morphological differentiation in murine neuroblastoma cells in culture. The percentage of differentiated cells depends on the concentration of dexamethasone in the medium and duration of treatment. After drug removal (with or without replating), many cells maintain their differentiated phenotype. Dexamethasone-treated cells have larger soma and contain more protein. Dexamethasone also produces a concentration-dependent inhibition of population growth. Growth inhibition is complete by 2 days following treatment with dexamethasone 50 micrograms/ml. Complete growth inhibition is achieved prior to the complete expression of morphological differentiation. Androstenedione, testosterone, and 17-beta-estradiol--all steroids without glucocorticoid activity--have no differentiating effect, although they inhibit growth or cause cell death at higher concentrations.
地塞米松可诱导培养的小鼠神经母细胞瘤细胞发生形态分化。分化细胞的百分比取决于培养基中地塞米松的浓度和处理时间。去除药物后（无论是否重新接种），许多细胞仍保持其分化表型。用地塞米松处理的细胞具有更大的细胞体且含有更多蛋白质。地塞米松还会对细胞群体生长产生浓度依赖性抑制。用50微克/毫升地塞米松处理2天后，生长抑制完全。在形态分化完全表达之前就实现了完全生长抑制。雄烯二酮、睾酮和17-β-雌二醇——所有无糖皮质激素活性的类固醇——均无分化作用，尽管它们在较高浓度下会抑制生长或导致细胞死亡。
