Kodama K, Doi O, Higashiyama M, Yokouchi H, Tateishi R, Mori Y
Department of Thoracic Surgery, Center for Adult Diseases, Osaka.
Jpn J Cancer Res. 1994 Apr;85(4):335-8. doi: 10.1111/j.1349-7006.1994.tb02363.x.
Two different pathways of differentiation were investigated in Ewing's sarcoma (ES) cell line, designated CADO-ES1, which has been established in our laboratory. This cell line was induced to differentiate and display a neural phenotype when treated with dibutyryl cyclic adenosine monophosphate or when cultured in serum-free medium (HB101). In these in vitro differentiation studies, two different phenotypes were demonstrated by light and electron microscopy. One phenotype, present in a major portion of the cell population, had long neurites in which microtubules were ultrastructurally demonstrated. The other one, present in a minor portion of the cell population, consisted of flat cells with many short processes. After differentiation in serum-free medium, tumorigenicity in nude mice or colony-forming efficiency in soft agar was strongly depressed. In the cells, N-myc, c-fos and c-src genes were not amplified, and although c-myc was amplified by up to 2-fold, depending on the culture conditions, this appeared to be unrelated to the changes of phenotype. When tumor cells were transplanted into nude mice, cartilage was formed. The cartilage was immunoreactive with the antibody for HLA-ABC, indicating that it was derived from the tumor cells, not from mouse tissue.
在我们实验室建立的尤因肉瘤（ES）细胞系CADO-ES1中，研究了两种不同的分化途径。当用二丁酰环磷酸腺苷处理或在无血清培养基（HB101）中培养时，该细胞系被诱导分化并呈现神经表型。在这些体外分化研究中，通过光学显微镜和电子显微镜证实了两种不同的表型。一种表型存在于大部分细胞群体中，具有长神经突，在超微结构上显示有微管。另一种表型存在于小部分细胞群体中，由具有许多短突起的扁平细胞组成。在无血清培养基中分化后，裸鼠的致瘤性或软琼脂中的集落形成效率被强烈抑制。在这些细胞中，N-myc、c-fos和c-src基因未扩增，尽管c-myc根据培养条件可扩增至2倍，但这似乎与表型变化无关。当将肿瘤细胞移植到裸鼠中时，形成了软骨。该软骨与HLA-ABC抗体发生免疫反应，表明它源自肿瘤细胞，而非小鼠组织。
