Meskar A, Le Bras R, L'Emeillat M, Ménez J F
J Clin Chem Clin Biochem. 1985 Apr;23(4):197-202. doi: 10.1515/cclm.1985.23.4.197.
A comparison of methods, two based upon 5-hydroxymethylfurfural determination (colorimetric using a semi-automated procedure and HPLC) and ion exchange chromatography (HbA1 using BioRad method) was performed. The influence of variables on 5-hydroxymethylfurfural determination is reported. The dialysis step appears to be essential for an accurate determination of 5-hydroxymethylfurfural released from glycated haemoglobin. The adjustment of haemoglobin concentration to an accurately known level is required for the colorimetric method, in order to achieve the same background colour absorbance. For the HPLC method this step is not necessary as 5-hydroxymethylfurfural liberation was proved to be stoichiometric. The Sep Pak C-18 cartridge purification appears to be an improvement for the accurate determination of 5-hydroxymethylfurfural using HPLC. When the three methods were compared the overlap between normals and diabetics was found to be less, using the 5-hydroxymethylfurfural HPLC method, suggesting that this procedure, although more time consuming, seems to be more useful in the management of diabetes mellitus.
对两种基于5-羟甲基糠醛测定的方法（使用半自动程序的比色法和高效液相色谱法）和离子交换色谱法（使用BioRad方法测定糖化血红蛋白）进行了比较。报告了变量对5-羟甲基糠醛测定的影响。透析步骤对于准确测定糖化血红蛋白释放的5-羟甲基糠醛似乎至关重要。比色法需要将血红蛋白浓度调整到准确已知的水平，以实现相同的背景颜色吸光度。对于高效液相色谱法，此步骤并非必要，因为已证明5-羟甲基糠醛的释放是化学计量的。Sep Pak C-18柱净化似乎改进了使用高效液相色谱法准确测定5-羟甲基糠醛的方法。当比较这三种方法时，发现使用5-羟甲基糠醛高效液相色谱法时，正常人和糖尿病患者之间的重叠较少，这表明该方法虽然更耗时，但在糖尿病管理中似乎更有用。
