Gruber C, Levine S
J Gen Virol. 1985 Jun;66 ( Pt 6):1241-7. doi: 10.1099/0022-1317-66-6-1241.
The cell-associated glycoproteins of respiratory syncytial virus included GP1 (90K), VP70 (70K), VGP48 (48K) and GP26 (26K). The time course of virus protein synthesis in HeLa cells revealed that the nucleocapsid protein (VPN41) was the first to appear at 11 h post-infection followed by the appearance of the other viral proteins at 16 h post-infection. Pulse-chase experiments with [3H]leucine or [35S]methionine demonstrated that the precursor for VGP48 and GP26 was an uncleaved protein of 70K (VP70) which required at least 30 min to chase into its final products, while the precursor for GP1 was a glycoprotein of 46K, and also required at least 30 min to chase into GP1. Trypsin treatment of monensin-treated infected cells suggested that VP70 can be cleaved intracellularly into its products VGP48 and GP26.
呼吸道合胞病毒的细胞相关糖蛋白包括GP1（90K）、VP70（70K）、VGP48（48K）和GP26（26K）。对HeLa细胞中病毒蛋白合成时间进程的研究表明，核衣壳蛋白（VPN41）在感染后11小时最先出现，随后其他病毒蛋白在感染后16小时出现。用[3H]亮氨酸或[35S]甲硫氨酸进行的脉冲追踪实验表明，VGP48和GP26的前体是一种未切割的70K蛋白（VP70），它至少需要30分钟才能追踪到其最终产物，而GP1的前体是一种46K的糖蛋白，也至少需要30分钟才能追踪到GP1。对莫能菌素处理的感染细胞进行胰蛋白酶处理表明，VP70可在细胞内切割成其产物VGP48和GP26。
