Elango N, Prince G A, Murphy B R, Venkatesan S, Chanock R M, Moss B
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1906-10. doi: 10.1073/pnas.83.6.1906.
A cDNA copy of the G glycoprotein gene of human respiratory syncytial virus (RSV) was placed under control of a vaccinia virus promoter and inserted into the thymidine kinase locus of the vaccinia virus genome. The recombinant vaccinia virus retained infectivity and expressed a 93-kDa protein that migrated with the authentic RSV G glycoprotein upon polyacrylamide gel electrophoresis. Glycosylation of the expressed protein and transport to the cell surface were demonstrated in the absence of other RSV proteins. Cotton rats that were inoculated intradermally with the infectious recombinant virus produced serum antibody to the G glycoprotein that neutralized RSV in vitro. Furthermore, the vaccinated animals were resistant to lower respiratory tract infection upon intranasal inoculation with RSV and had reduced titers of RSV in the nose.
将人类呼吸道合胞病毒（RSV）G糖蛋白基因的互补DNA（cDNA）拷贝置于痘苗病毒启动子的控制之下，并插入痘苗病毒基因组的胸苷激酶基因座。重组痘苗病毒保留了感染性，并表达了一种93 kDa的蛋白质，该蛋白质在聚丙烯酰胺凝胶电泳中与天然RSV G糖蛋白迁移情况相同。在没有其他RSV蛋白的情况下，证实了表达蛋白的糖基化以及向细胞表面的转运。经皮内接种感染性重组病毒的棉鼠产生了针对G糖蛋白的血清抗体，该抗体在体外可中和RSV。此外，接种疫苗的动物经鼻内接种RSV后对下呼吸道感染具有抵抗力，且鼻腔内RSV滴度降低。
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