Meier Felix, Williams Tom, Paulsen Ian
School of Natural Sciences, Macquarie University, Sydney 2109, Australia.
ARC Centre of Excellence in Synthetic Biology, Sydney 2109, Australia.
Bioinform Adv. 2025 Mar 4;5(1):vbaf038. doi: 10.1093/bioadv/vbaf038. eCollection 2025.
Welly is a web-based tool designed to simplify the visualization and analysis of growth curves from 96- and 384-well plates, addressing the limitations of existing commercial and coding-based solutions. Users can upload plate reader data in CSV or Excel format, easily select sample names and replicates and Welly generates interactive growth curves displaying the mean and standard deviation of triplicates. Additional features include heat map visualizations of maximum values, and downloadable interactive graphs of publication-quality figures and statistics files containing area under curve and max growth rate value of replicates.
Welly is freely available at https://synbioexplorer.pythonanywhere.com, providing an easy-to-use interface accessible to all. All the code is publicly available at the github repository https://github.com/SynBioExplorer/Welly under the MIT license. The website will remain freely accessible for at least 2 years post publication, likely longer.
Welly是一个基于网络的工具，旨在简化对96孔板和384孔板生长曲线的可视化和分析，解决现有商业和基于编码的解决方案的局限性。用户可以上传CSV或Excel格式的酶标仪数据，轻松选择样本名称和重复样本，Welly会生成交互式生长曲线，显示一式三份样本的平均值和标准差。其他功能包括最大值的热图可视化，以及可下载的具有出版质量的交互式图形和统计文件，其中包含曲线下面积和重复样本的最大生长速率值。
