Department of Biology, Institute of Microbiology, ETH Zurich, 8093, Zurich, Switzerland.
BMC Bioinformatics. 2024 Feb 12;25(1):67. doi: 10.1186/s12859-024-05692-y.
Recording and analyzing microbial growth is a routine task in the life sciences. Microplate readers that record dozens to hundreds of growth curves simultaneously are increasingly used for this task raising the demand for their rapid and reliable analysis.
Here, we present Dashing Growth Curves, an interactive web application ( http://dashing-growth-curves.ethz.ch/ ) that enables researchers to quickly visualize and analyze growth curves without the requirement for coding knowledge and independent of operating system. Growth curves can be fitted with parametric and non-parametric models or manually. The application extracts maximum growth rates as well as other features such as lag time, length of exponential growth phase and maximum population size among others. Furthermore, Dashing Growth Curves automatically groups replicate samples and generates downloadable summary plots for of all growth parameters.
Dashing Growth Curves is an open-source web application that reduces the time required to analyze microbial growth curves from hours to minutes.
记录和分析微生物生长是生命科学中的一项常规任务。越来越多的微孔板读数器用于同时记录数十到数百个生长曲线，这提高了对其快速可靠分析的需求。
在这里，我们介绍了 Dashing Growth Curves，这是一个交互式网络应用程序（http://dashing-growth-curves.ethz.ch/），它使研究人员能够快速可视化和分析生长曲线，而无需编码知识，并且与操作系统无关。可以使用参数和非参数模型对生长曲线进行拟合，也可以手动进行拟合。该应用程序提取最大生长速率以及其他特征，例如滞后时间、指数生长阶段的长度和最大种群大小等。此外，Dashing Growth Curves 会自动对重复样本进行分组，并为所有生长参数生成可下载的摘要图。
Dashing Growth Curves 是一个开源的网络应用程序，可将分析微生物生长曲线所需的时间从数小时缩短到数分钟。
