Irani Soussan, Alimohammadi Shohreh, Ataei Fatemeh
Dept. of Oral Pathology, Dental Research Centre, Dental Faculty, Hamadan University of Medical Sciences, Hamadan, Iran. Lecturer at Griffith University, Gold Coast, Australia.
Gynecologist and Perinatologist, Hamadan University of Medical Sciences, Hamadan, Iran.
J Dent (Shiraz). 2025 Mar 1;26(1):55-60. doi: 10.30476/dentjods.2024.100219.2202. eCollection 2025 Mar.
Tooth development is initiated by localized thickenings of the primary epithelial bands. Dentin sialophosphoprotein (DSPP) is the most abundant non-collagenous protein in the dentin matrix. DSPP's expression extends to multiple tissues, including dentin, cementum, and bone. However, the role of DSPP is not yet clear in the development of human tooth.
The current study aimed to examine the DSPP expression profile during the development of human primary tooth structures with a sufficiently large sample size.
In this experimental study, a total of 33 samples of aborted human fetuses were divided into three age groups <16 weeks, <19 weeks and ≥19 weeks. Immunohistochemistry (IHC) was performed with Anti-DSPP rabbit polyclonal antibody. A two-way ANOVA analysis was conducted to examine the differences between the groups (< 0.05).
An increase in DSPP expression was observed with the progression of gestational weeks in different histological structures of developing human primary teeth.
An increase in the expression level of DSPP with the progression of gestational weeks may confirm that reciprocal interactions between the enamel organ (EO) and dental pulp cells contribute to tooth formation.
牙齿发育由原发性上皮带的局部增厚启动。牙本质涎磷蛋白（DSPP）是牙本质基质中最丰富的非胶原蛋白。DSPP的表达延伸至多种组织，包括牙本质、牙骨质和骨。然而，DSPP在人类牙齿发育中的作用尚不清楚。
本研究旨在通过足够大的样本量检测人类乳牙结构发育过程中DSPP的表达谱。
在本实验研究中，共将33例流产的人类胎儿样本分为三个年龄组：<16周、<19周和≥19周。使用抗DSPP兔多克隆抗体进行免疫组织化学（IHC）。进行双向方差分析以检验组间差异（<0.05）。
随着孕周增加，在发育中的人类乳牙不同组织结构中观察到DSPP表达增加。
随着孕周增加DSPP表达水平升高可能证实釉器（EO）与牙髓细胞之间的相互作用有助于牙齿形成。
