Martinod S, Brossard M, Moreau Y
J Parasitol. 1985 Jun;71(3):269-73.
Previous epidemiological studies allowed us to accurately define endemic areas of canine babesiosis and tick distribution in southeastern France (Martinod, 1983). Using a micro-ELISA test 100 dogs sera were tested with 3 antigens: Babesia canis, Dermacentor reticulatus and Ixodes ricinus. Antibodies against B. canis and its vector D. reticulatus were detected in an endemic area, sometimes with high levels (optical density 1.38 and 0.80 respectively). A correlation factor and regression lines were found between ELISA activity of B. canis and vector tick antigens, even for dogs which never showed any babesiosis symptoms. These results were compared with those of an area without any babesiosis. Furthermore I. ricinus antigens detected ELISA activity in sera of dogs; some cross reactions were observed between I. ricinus and D. reticulatus antigen.
以往的流行病学研究使我们能够准确界定法国东南部犬巴贝斯虫病的流行区域以及蜱的分布情况（Martinod，1983年）。使用微量ELISA检测法，用3种抗原对100份犬血清进行检测：犬巴贝斯虫、血红扇头蜱和蓖麻硬蜱。在一个流行区域检测到了针对犬巴贝斯虫及其传播媒介血红扇头蜱的抗体，有时抗体水平较高（光密度分别为1.38和0.80）。即使对于从未表现出任何巴贝斯虫病症状的犬只，也发现了犬巴贝斯虫和媒介蜱抗原的ELISA活性之间的相关系数和回归线。将这些结果与无任何巴贝斯虫病的区域的结果进行了比较。此外，蓖麻硬蜱抗原在犬血清中检测到了ELISA活性；在蓖麻硬蜱和血红扇头蜱抗原之间观察到了一些交叉反应。
