Zuo Yanhai, Xiao Shouyun
Department of Orthopedics, Sijing Hospital of Songjiang District, Shanghai, China.
Department of Orthopedics, BenQ Medical Center, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, China.
Anat Cell Biol. 2025 Jun 30;58(2):166-172. doi: 10.5115/acb.24.306. Epub 2025 Mar 18.
A good vascular perfusion is vital for the vessel related cadaver surgical training (CST). The objective of this study was to (1) explore the feasibility of using lard as a vascular filler in vascular perfusion in fresh cadavers and (2) explore a simple and environmentally-friendly method for the cadaveric preparation in CST. Ten fresh pig cadavers were employed for separation of lard, and the following vascular perfusion. At about 30°C-40°C, the dye was added to the lard to obtain a satisfactory lard-dye mixture. Thereafter, this mixture was slowly infused into the aorta abdominalis and the lateral saphenous vein. After infusion, the specimens, together with some ice bags, were stored in foam boxes for 3-4 hours. The vascular perfusion of the bones, the muscles, the skin, and the viscus was carefully observed. The lard and the dye mixed well and solidified at 4°C. The time for the cadaveric preparation via this method was approximately 5 hours. The success rate of this method was 100%. Both the arteries and the veins of the bones, the muscles, the skin, and the viscus could be successfully infused at room temperature. Notably, even the capillary network is infused very clearly. After being placed at 0°C-4°C for 3-4 hours, the infused lard solidified. The lard-based vascular injection technique is simple, thermo-responsive and environmentally-friendly. This technique allows CST to be independent of perfusion techniques, equipment, and location.
良好的血管灌注对于与血管相关的尸体外科训练(CST)至关重要。本研究的目的是:(1)探索在新鲜尸体的血管灌注中使用猪油作为血管填充剂的可行性;(2)探索一种用于CST中尸体准备的简单且环保的方法。使用10具新鲜猪尸体分离猪油并进行如下血管灌注。在约30°C - 40°C时,将染料加入猪油中以获得令人满意的猪油 - 染料混合物。此后,将该混合物缓慢注入腹主动脉和大隐静脉。注入后,将标本连同一些冰袋一起储存在泡沫箱中3 - 4小时。仔细观察骨骼、肌肉、皮肤和内脏的血管灌注情况。猪油和染料混合良好并在4°C时凝固。通过这种方法进行尸体准备的时间约为5小时。该方法的成功率为100%。在室温下,骨骼、肌肉、皮肤和内脏的动脉和静脉均可成功注入。值得注意的是,甚至毛细血管网络也灌注得非常清晰。在0°C - 4°C放置3 - 4小时后,注入的猪油凝固。基于猪油的血管注射技术简单、热响应性好且环保。该技术使CST能够独立于灌注技术、设备和地点。