Gamelli R L, Hebert J C, Foster R S
J Trauma. 1985 Jul;25(7):615-9. doi: 10.1097/00005373-198507000-00007.
Using a model of uncomplicated burn injury in mice, we assayed the bone marrow and splenic production of granulocytes and macrophages after burn injury. The effects of burn size and burn wound excision and closure were studied. Using an in vitro quantitative clonal culture technique for granulocyte/macrophage progenitor cells (GM-CFC), myeloid precursors were directly assayed. Burns of a 10% body surface area were equal to burns of larger magnitude for effects on marrow and splenic granulocyte/macrophage production. The total peripheral blood leukocyte and lymphocyte counts were depressed at days 1 and 4 postburn but were elevated at days 8 and 12. Granulocytes, however, remained significantly increased at days 8 and 12. The bone marrow response to burning showed an initial depression in marrow cellularity on day 1 with return to normal values by days 8 and 12. The numbers of GM-CFC were significantly elevated on days 4-12 with a near threefold increase in the number of GM-CFC 12 days following burn injury. The splenic response to burn injury was characterized by a decrease in the splenic index on day 1 but then a persistent increase at days 8 and 12. Total splenic cellularity was depressed on day 1 but significantly increased at days 8 and 12. The total number of splenic GM-CFC was increased on days 4-12 with a 100-fold increase on day 8. The immediate or delayed excision of the burn wound did not alter marrow or splenic response to burning. We conclude that following a cutaneous injury there is a marked alteration in the generation of the phagocytic cells of the granulocyte and macrophage series and that this response is secondary to the wounding process.
利用小鼠单纯性烧伤模型，我们检测了烧伤后骨髓和脾脏中粒细胞及巨噬细胞的生成情况。研究了烧伤面积以及烧伤创面切除与闭合的影响。采用粒细胞/巨噬细胞祖细胞（GM-CFC）的体外定量克隆培养技术，直接检测髓系前体细胞。10%体表面积的烧伤对骨髓和脾脏粒细胞/巨噬细胞生成的影响与更大面积的烧伤相同。烧伤后第1天和第4天，外周血白细胞和淋巴细胞总数降低，但在第8天和第12天升高。然而，粒细胞在第8天和第12天仍显著增加。骨髓对烧伤的反应表现为第1天骨髓细胞数量最初减少，到第8天和第12天恢复正常。GM-CFC数量在第4 - 12天显著升高，烧伤后12天GM-CFC数量增加近三倍。脾脏对烧伤的反应特点是第1天脾脏指数下降，但在第8天和第12天持续升高。脾脏细胞总数在第1天减少，但在第8天和第12天显著增加。脾脏GM-CFC总数在第4 - 12天增加，第8天增加100倍。烧伤创面的即时或延迟切除并未改变骨髓或脾脏对烧伤的反应。我们得出结论，皮肤损伤后，粒细胞和巨噬细胞系列吞噬细胞的生成发生显著改变，且这种反应继发于创伤过程。
