Zhao Ling, Qi Jianzhao
Department of Pharmacy, School of Medicine, Xi'an International University, Xi'an, China.
College of Chemistry & Pharmacy, Shaanxi Key Laboratory of Natural Products & Chemical Biology, Northwest A&F University, Yangling, China.
Mitochondrial DNA B Resour. 2025 Mar 13;10(4):292-297. doi: 10.1080/23802359.2025.2478128. eCollection 2025.
(Berk. & M.A.Curtis) Donk 1933, a globally renowned white-rot basidiomycete belonging to the Polyporales order, holds significant potential for lignin degradation, yet its mitochondrial genome has received comparatively little attention. Our study concentrates on a specimen designated NEFU_01, sourced from the Forest Botanical Garden in Heilongjiang Province, China. Utilizing next-generation sequencing (NGS) technology, we have successfully delineated the complete mitochondrial genome of this isolate. The genome is composed of 15 protein-coding genes (PCGs), an array of 24 transfer RNAs (tRNAs), and a pair of ribosomal RNAs (rRNAs), encompassing a total of 163,380 base pairs (bp). Additionally, the genome encodes 28 LAGLIDADG- and 10 GIY-YIG-homing endonucleases. The nucleotide composition is characterized by adenine (A) at 37.02%, cytosine (C) at 12.91%, guanine (G) at 13.04%, and thymine (T) at 37.03%, culminating in a GC content of 25.95%. Subsequently, we undertook a phylogenetic analysis, employing a dataset of 25 mitochondrial genomes to construct a phylogenetic tree. This research represents the first comprehensive foray into understanding the phylogenetic relationships of with its Basidiomycete kin, particularly its sister-group relationship with Fr. (1821), thereby laying a substantive groundwork for subsequent evolutionary and taxonomic studies within this mycological cohort.
（伯克和M.A.柯蒂斯）唐克1933年，一种全球知名的属于多孔菌目的白腐担子菌，在木质素降解方面具有巨大潜力，但其线粒体基因组受到的关注相对较少。我们的研究集中于一个名为NEFU_01的标本，它来自中国黑龙江省的森林植物园。利用下一代测序（NGS）技术，我们成功描绘了该分离株的完整线粒体基因组。该基因组由15个蛋白质编码基因（PCG）、一系列24个转运RNA（tRNA）和一对核糖体RNA（rRNA）组成，总共包含163,380个碱基对（bp）。此外，该基因组编码28个LAGLIDADG和10个GIY - YIG归巢内切酶。核苷酸组成的特点是腺嘌呤（A）占37.02%，胞嘧啶（C）占12.91%，鸟嘌呤（G）占13.04%，胸腺嘧啶（T）占37.03%，GC含量为25.95%。随后，我们进行了系统发育分析，使用25个线粒体基因组的数据集构建了一棵系统发育树。这项研究是首次全面探索了解其与担子菌亲属的系统发育关系，特别是它与弗里斯（1821年）的姐妹群关系，从而为该真菌类群内后续的进化和分类研究奠定了坚实基础。
