• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

解析PRKCI及关键癌症相关基因在乳腺癌发生和转移中的作用。

Unravelling the role of PRKCI and key-cancer related genes in breast cancer development and metastasis.

作者信息

Shah Hania, Khan Khushbukhat, Badshah Yasmin, Trembley Janeen H, Ashraf Naeem Mahmood, Shabbir Maria, Afsar Tayyaba, Aldisi Dara, Khan Dilawer, Razak Suhail

机构信息

Department of Healthcare Biotechnology, Atta-Ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan.

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, USA.

出版信息

Discov Oncol. 2025 Mar 18;16(1):350. doi: 10.1007/s12672-025-02133-x.

DOI:10.1007/s12672-025-02133-x
PMID:40100546
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11920535/
Abstract

BACKGROUND

Breast cancer is one of the most common causes of fatalities in females globally. Rising cases of drug resistance against existing chemotherapeutics are great problem. To address this issue, there is a need to find appropriate biomarker that could be used to detect cancer at early stages, so drug resistance development can be avoided. Protein Kinase C iota (PKCɩ), an AGC kinase, has an oncogenic role in cancers and its expression and Single nucleotide polymorphisms (SNPs) have been reported to be associated with the cancer development. So, the study aims were to examine the expression of PKCɩ, Protein Kinase B (AKT), Suppressor of cytokine signaling 3 (SOCS3), Vascular endothelial growth factor (VEGF), Krupple like factor 3 (KLF3), Tumor protein D52 (TPD52), Hypoxia inducible factor (HIF1α) and microRNA-124 (miR-124) in breast cancer and association of PKCɩ variants (G34W & F66Y) with breast cancer.

METHODS

Genetic expression assay was performed through real time Polymerase Chain reaction (PCR), whereas the genotypic association of PKCɩ SNPs with breast cancer was accomplished through Tetra-ARMS PCR.

RESULTS

The expression levels of PKCɩ, AKT, SOC3, VEGF, HIF1α and TPD52 were elevated in patients as compared to control whereas the expression levels of miR-124 and KLF3 were lowered in patients. Positive association of variant G34W (TT) of PKCɩ with breast cancer has been explored through ARM's PCR, while no association of variant F66Y with breast cancer was found.

CONCLUSION

Hence, the results suggest that PKCɩ and related genes can have a role in breast cancer and after further verification can serve as the potential biomarkers for the early-diagnosis and prognosis of breast cancer.

摘要

背景

乳腺癌是全球女性死亡的最常见原因之一。对现有化疗药物耐药性病例的增加是一个重大问题。为解决这一问题，需要找到合适的生物标志物，用于在早期检测癌症，从而避免耐药性的发展。蛋白激酶Cι（PKCι）是一种AGC激酶，在癌症中具有致癌作用，其表达和单核苷酸多态性（SNP）据报道与癌症发展相关。因此，本研究旨在检测蛋白激酶Cι（PKCι）、蛋白激酶B（AKT）、细胞因子信号转导抑制因子3（SOCS3）、血管内皮生长因子（VEGF）、类 Krupple 因子3（KLF3）、肿瘤蛋白D52（TPD52）、缺氧诱导因子（HIF1α）和微小RNA-124（miR-124）在乳腺癌中的表达情况，以及PKCι变体（G34W和F66Y）与乳腺癌的关联。

方法

通过实时聚合酶链反应（PCR）进行基因表达检测，而PKCι单核苷酸多态性与乳腺癌的基因型关联则通过四引物扩增受阻突变体系PCR完成。

结果

与对照组相比，患者中PKCι、AKT、SOC3、VEGF、HIF1α和TPD52的表达水平升高，而miR-124和KLF（3）的表达水平降低。通过扩增受阻突变体系PCR研究发现PKCι变体G34W（TT）与乳腺癌呈正相关，而未发现变体F66Y与乳腺癌有关联。

结论

因此，结果表明PKCι和相关基因可能在乳腺癌中起作用，经进一步验证后可作为乳腺癌早期诊断和预后的潜在生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/e827e8ce61ca/12672_2025_2133_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/676676264f7b/12672_2025_2133_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/f08ad903b939/12672_2025_2133_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/e991a0cd1209/12672_2025_2133_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/b6f6ead5c03c/12672_2025_2133_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/1d761962f694/12672_2025_2133_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/36e6e630b1f0/12672_2025_2133_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/d1cbe053051b/12672_2025_2133_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/2c4bca8e3488/12672_2025_2133_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/e827e8ce61ca/12672_2025_2133_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/676676264f7b/12672_2025_2133_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/f08ad903b939/12672_2025_2133_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/e991a0cd1209/12672_2025_2133_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/b6f6ead5c03c/12672_2025_2133_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/1d761962f694/12672_2025_2133_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/36e6e630b1f0/12672_2025_2133_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/d1cbe053051b/12672_2025_2133_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/2c4bca8e3488/12672_2025_2133_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a95/11920535/e827e8ce61ca/12672_2025_2133_Fig9_HTML.jpg

相似文献

1
Unravelling the role of PRKCI and key-cancer related genes in breast cancer development and metastasis.解析PRKCI及关键癌症相关基因在乳腺癌发生和转移中的作用。
Discov Oncol. 2025 Mar 18;16(1):350. doi: 10.1007/s12672-025-02133-x.
2
Impact of deleterious missense PRKCI variants on structural and functional dynamics of protein.PRKCI 种系有害错义变体对蛋白结构和功能动态的影响。
Sci Rep. 2022 Mar 8;12(1):3781. doi: 10.1038/s41598-022-07526-4.
3
Unravelling Structure, Localization, and Genetic Crosstalk of KLF3 in Human Breast Cancer.解析 KLF3 在人乳腺癌中的结构、定位和遗传串扰。
Biomed Res Int. 2020 Dec 28;2020:1354381. doi: 10.1155/2020/1354381. eCollection 2020.
4
Tumor-suppressive microRNA-34a inhibits breast cancer cell migration and invasion via targeting oncogenic TPD52.肿瘤抑制性微小RNA-34a通过靶向致癌蛋白TPD52抑制乳腺癌细胞的迁移和侵袭。
Tumour Biol. 2016 Jun;37(6):7481-91. doi: 10.1007/s13277-015-4623-4. Epub 2015 Dec 17.
5
Deletion of histidine triad nucleotide-binding protein 1/PKC-interacting protein in mice enhances cell growth and carcinogenesis.小鼠中组氨酸三联体核苷酸结合蛋白1/蛋白激酶C相互作用蛋白的缺失会增强细胞生长和致癌作用。
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7824-9. doi: 10.1073/pnas.1332160100. Epub 2003 Jun 16.
6
Predicting 3D Structure, Cross Talks, and Prognostic Significance of in Cervical Cancer.预测宫颈癌中[具体内容未给出]的三维结构、相互作用及预后意义。
Front Oncol. 2022 Jan 3;11:797007. doi: 10.3389/fonc.2021.797007. eCollection 2021.
7
Cross talk of tumor protein D52 (TPD52) with KLF9, PKCε, and MicroRNA 223 in ovarian cancer.卵巢癌细胞中肿瘤蛋白 D52（TPD52）与 KLF9、PKCε 和 MicroRNA 223 的串扰。
J Ovarian Res. 2023 Oct 13;16(1):202. doi: 10.1186/s13048-023-01292-1.
8
Genetic Effects of Vascular Endothelial Growth Factor A (VEGF-A) and Its Association with Disease Progression in Breast Cancer Population of Saudi Arabia.血管内皮生长因子A（VEGF-A）的遗传效应及其与沙特阿拉伯乳腺癌人群疾病进展的关联
Asian Pac J Cancer Prev. 2020 Jan 1;21(1):139-145. doi: 10.31557/APJCP.2020.21.1.139.
9
Effect of protein kinase C inhibitor (PKCI) on radiation sensitivity and c-fos transcription.蛋白激酶C抑制剂（PKCI）对辐射敏感性和c-fos转录的影响。
Int J Radiat Oncol Biol Phys. 2001 Feb 1;49(2):397-405. doi: 10.1016/s0360-3016(00)01485-1.
10
Associations of Single Nucleotide Polymorphism in miR-146a Gene with Susceptibility to Breast Cancer in the Iranian Female.miR-146a 基因单核苷酸多态性与伊朗女性乳腺癌易感性的关联。
Asian Pac J Cancer Prev. 2020 Jun 1;21(6):1585-1593. doi: 10.31557/APJCP.2020.21.6.1585.

本文引用的文献

1
KLF3 promotes colorectal cancer growth by activating WNT1.KLF3 通过激活 WNT1 促进结直肠癌生长。
Aging (Albany NY). 2024 Feb 1;16(3):2475-2493. doi: 10.18632/aging.205494.
2
Pan-cancer analysis of Krüppel-like factor 3 and its carcinogenesis in pancreatic cancer.泛癌症分析 Krüppel 样因子 3 及其在胰腺癌中的致癌作用。
Front Immunol. 2023 Aug 3;14:1167018. doi: 10.3389/fimmu.2023.1167018. eCollection 2023.
3
Androgen receptor suppresses lung cancer invasion and increases cisplatin response decreasing TPD52 expression.
雄激素受体抑制肺癌侵袭并增加顺铂反应，降低 TPD52 表达。
Int J Biol Sci. 2023 Jul 16;19(12):3709-3725. doi: 10.7150/ijbs.84577. eCollection 2023.
4
SOCS3 protein expression predicts the responses of advanced non-small cell lung cancer patients to platinum-based chemotherapy.SOCS3蛋白表达可预测晚期非小细胞肺癌患者对铂类化疗的反应。
Ann Transl Med. 2023 Jan 31;11(2):94. doi: 10.21037/atm-22-6065.
5
Impact of deleterious missense PRKCI variants on structural and functional dynamics of protein.PRKCI 种系有害错义变体对蛋白结构和功能动态的影响。
Sci Rep. 2022 Mar 8;12(1):3781. doi: 10.1038/s41598-022-07526-4.
6
Influence of PRKCE non-synonymous variants on protein dynamics and functionality.PRKCE 非同义变异对蛋白质动力学和功能的影响。
Hum Mol Genet. 2022 Jul 7;31(13):2236-2261. doi: 10.1093/hmg/ddac029.
7
Resveratrol reduces drug resistance of SCLC cells by suppressing the inflammatory microenvironment and the STAT3/VEGF pathway.白藜芦醇通过抑制炎症微环境和STAT3/VEGF通路降低小细胞肺癌细胞的耐药性。
FEBS Open Bio. 2021 Aug;11(8):2256-2265. doi: 10.1002/2211-5463.13230. Epub 2021 Jul 16.
8
Sequencing and Characterization of Mitochondrial Protein-Coding Genes for (Cypriniformes: Cyprinidae) with Phylogenetic Consideration.线粒体蛋白编码基因的测序和特征分析（鲤形目：鲤科）及其系统发育考虑。
Biomed Res Int. 2020 Dec 7;2020:5980135. doi: 10.1155/2020/5980135. eCollection 2020.
9
Unravelling Structure, Localization, and Genetic Crosstalk of KLF3 in Human Breast Cancer.解析 KLF3 在人乳腺癌中的结构、定位和遗传串扰。
Biomed Res Int. 2020 Dec 28;2020:1354381. doi: 10.1155/2020/1354381. eCollection 2020.
10
Hypoxia-induced therapy resistance: Available hypoxia-targeting strategies and current advances in head and neck cancer.缺氧诱导的治疗耐药性：头颈部癌中现有的缺氧靶向策略及当前进展
Transl Oncol. 2021 Mar;14(3):101017. doi: 10.1016/j.tranon.2021.101017. Epub 2021 Jan 16.