Department of Genetic, Collage of Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran.
Asian Pac J Cancer Prev. 2020 Jun 1;21(6):1585-1593. doi: 10.31557/APJCP.2020.21.6.1585.
MicroRNAs (miRNAs), short regulatory RNAs, function as negative regulators able to modulate gene expression. Just as other genetic variant, single nucleotide polymorphisms (SNPs) in miRNA genes, may have an impact on their expression and/or maturation and hence leading to different consequences in carcinogenesis. Accordingly, this study aimed to assess the frequency of miR-146a G/C (rs2910164) polymorphism and its association with susceptibility to breast cancer in Iranian women.
We conducted a case-control study using Tetra ARMS polymerase chain reaction (Tetra ARMS PCR) method in 100 Iranian female participants (50 breast cancer patients and 50 controls). Besides, a number of sequenced samples were chosen to confirm the accuracy of genotyping by Tetra ARMA PCR. SPSS software was utilized for all statistical analyses. The odds ratios (ORs) and 95% confidence intervals (95% CIs) were applied to analyze the association between the SNP frequency and breast cancer.
The frequency of genotypes for G/G, G/C, and C/C were 23 (46%), 26 (52%), and 1 (2%) among cases and 15 (30%), 33 (66%), and 2(4%) among controls, respectively. The results generated by the groups did not show any significant correlation between miR-146a G/C (rs2910164) polymorphism and breast cancer, either at genotype or allele levels (P>0.05). F-SNP-based in silico analysis indicated possible modifications in transcriptional regulations induced by miR-146a G/C (rs2910164) variations.
Overall, our results indicated no correlation between miR-146a G/C (rs2910164) polymorphism and genetic susceptibility to breast cancer in Iranian female populations. However, these findings need to be further confirmed by analyses of a larger number of cases.
微小 RNA（miRNAs）是一种短的调节 RNA，作为负调控因子发挥作用，能够调节基因表达。就像其他遗传变异一样，miRNA 基因中的单核苷酸多态性（SNP）可能会影响其表达和/或成熟，从而导致致癌作用的不同后果。因此，本研究旨在评估 miR-146a G/C（rs2910164）多态性的频率及其与伊朗女性乳腺癌易感性的关系。
我们使用 Tetra ARMS 聚合酶链反应（Tetra ARMS PCR）方法对 100 名伊朗女性参与者（50 名乳腺癌患者和 50 名对照者）进行了病例对照研究。此外，选择了一些测序样本来确认 Tetra ARMA PCR 基因分型的准确性。所有统计分析均使用 SPSS 软件进行。应用优势比（OR）和 95%置信区间（95%CI）分析 SNP 频率与乳腺癌之间的关系。
病例组的基因型 G/G、G/C 和 C/C 的频率分别为 23（46%）、26（52%）和 1（2%），对照组分别为 15（30%）、33（66%）和 2（4%）。两组结果均显示 miR-146a G/C（rs2910164）多态性与乳腺癌在基因型或等位基因水平上无显著相关性（P＞0.05）。基于 F-SNP 的计算机分析表明，miR-146a G/C（rs2910164）变异可能导致转录调控的改变。
总体而言，我们的结果表明 miR-146a G/C（rs2910164）多态性与伊朗女性乳腺癌遗传易感性之间无相关性。然而，这些发现需要通过对更多病例的分析进一步证实。
