Chen Ying-Wen, Su Yu-Cheng, Chen Wei-Yu, Wu Jer-Horng, Chen Jenn-Wei, Su Shu-Li, Chen Chang-Shi, Tsai Pe-Fang, Ko Wen-Chien, Chen Po-Lin
Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
Microb Drug Resist. 2025 Apr;31(4):97-106. doi: 10.1089/mdr.2024.0212. Epub 2025 Mar 18.
is prevalent in aquatic environments in Taiwan and known for its notable antimicrobial resistance. However, comprehensive pan-genomic studies for this species in Taiwan are limited. This study analyzed 28 clinical isolates using single-molecule real-time sequencing technology, coupled with diverse databases, to elucidate the whole genomes. The focus was on phylogenetic relatedness, antimicrobial resistance genes, and mobile genetic elements. Genomic analysis and multilocus sequence typing were utilized to identify strains of heterogeneous origins. The detection of various β-lactamase genes (, , , , , , and ) in clinical isolates raises concern, especially considering the use of carbapenems and third-generation cephalosporins in patients with severe infections. Notably, most strains carry chromosome-encoded β-lactamases, including AmpC, metallo-β-lactamase, and oxacillinase, and were susceptible to cefepime in drug susceptibility tests. strains were also susceptible to aminoglycosides, fluoroquinolones, tigecycline, and trimethoprim/sulfamethoxazole. Three of the 28 isolates carried plasmids containing an array of drug resistance genes, suggesting this species is likely a recipient or donor of drug resistance genes through horizontal gene transfer. Our findings provide valuable insights into the antimicrobial resistance of , highlighting the medical implications of its β-lactamase diversity and its potential role in the horizontal gene transfer of drug resistance genes.
在台湾的水生环境中普遍存在，并且以其显著的抗菌耐药性而闻名。然而，台湾针对该物种的全面泛基因组研究有限。本研究使用单分子实时测序技术并结合多种数据库，对28株临床分离株进行分析，以阐明其全基因组。重点关注系统发育相关性、抗菌耐药基因和可移动遗传元件。利用基因组分析和多位点序列分型来鉴定不同来源的菌株。临床分离株中检测到各种β-内酰胺酶基因（、、、、、和）令人担忧，尤其是考虑到在严重感染患者中使用碳青霉烯类和第三代头孢菌素。值得注意的是，大多数菌株携带染色体编码的β-内酰胺酶，包括AmpC、金属β-内酰胺酶和苯唑西林酶，并且在药敏试验中对头孢吡肟敏感。菌株对氨基糖苷类、氟喹诺酮类、替加环素和甲氧苄啶/磺胺甲恶唑也敏感。28株分离株中有3株携带含有一系列耐药基因的质粒，表明该物种可能是耐药基因通过水平基因转移的受体或供体。我们的研究结果为该菌的抗菌耐药性提供了有价值的见解，突出了其β-内酰胺酶多样性的医学意义及其在耐药基因水平基因转移中的潜在作用。
