Wu Yujie, Liu Cuihong, Liu Jinhua, Wang Wenwen, Qin Bixuan, Liu Honglei
Department of Ophthalmology, Xi'an People's Hospital (Xi'an Fourth Hospital), Xi'an 710004, Shaanxi Province, People's Republic of China.
Biomed Mater. 2025 Mar 27;20(3). doi: 10.1088/1748-605X/adc220.
This study aimed to investigate the osteogenic function of polyetheretherketone (PEEK) scaffolds modified with bone morphogenetic protein 2 (BMP2) and its possibility for orbital fracture repair. The 3D-printed PEEK sheets were combined with BMP2-loaded hyaluronic acid hydrogel (HAH) to fabricate PEEK-BMP2-HAH composite scaffolds. Bone marrow mesenchymal stem cells (BMSCs) were seeded onto PEEK or PEEK-BMP2-HAH scaffolds. Cell adhesion and cell proliferation were measured by transmission electron microscopy and CCK-8 assay. Alkaline phosphatase (ALP) chromogenic, alizarine red S staining, and PCR analysis of Runt-related transcription factor 2 (Runx2), collagen-I (Col-I), Osterix, and osteopontin (OPN) were performed to assess osteogenic activity. The rat orbital fracture defect model is proposed for evaluating the biocompatibility, osteogenic integration, and functional recovery of PEEK orbital implants. Compared with PEEK, cell adhesion and cell proliferation were increased in PEEK-BMP2-HAH scaffolds. ALP activity and mineralized nodule formation were increased in PEEK-BMP2-HAH scaffolds than that in PEEK the mRNA expression of Runx2, Osterix, Col-I and OPN was increased on PEEK-BMP2-HAH scaffolds than that on PEEK at 14 d of osteogenic induction. Besides, a bone defect animal model revealed that BMP2-HAH-modified PEEK scaffolds could effectively facilitate the repair of the orbital bone defect, with increased expression of OPN and Runx2. BMP2-loaded HAH effectively increased adhesion, proliferation, and osteogenic differentiation of BMSCs on PEEK. PEEK-BMP2-HAH scaffolds are expected to become new materials for orbital fracture repair.
本研究旨在探讨经骨形态发生蛋白2（BMP2）修饰的聚醚醚酮（PEEK）支架的成骨功能及其用于眼眶骨折修复的可能性。将3D打印的PEEK片材与负载BMP2的透明质酸水凝胶（HAH）相结合，制备PEEK-BMP2-HAH复合支架。将骨髓间充质干细胞（BMSCs）接种到PEEK或PEEK-BMP2-HAH支架上。通过透射电子显微镜和CCK-8法检测细胞黏附和细胞增殖情况。进行碱性磷酸酶（ALP）显色、茜素红S染色以及对 runt相关转录因子2（Runx2）、I型胶原（Col-I）、osterix和骨桥蛋白（OPN）的PCR分析，以评估成骨活性。提出大鼠眼眶骨折缺损模型，用于评估PEEK眼眶植入物的生物相容性、成骨整合和功能恢复情况。与PEEK相比，PEEK-BMP2-HAH支架上的细胞黏附和细胞增殖增加。PEEK-BMP2-HAH支架上的ALP活性和矿化结节形成比PEEK上的增加，在成骨诱导14天时，PEEK-BMP2-HAH支架上Runx2、osterix、Col-I和OPN的mRNA表达比PEEK上的增加。此外，骨缺损动物模型显示，BMP2-HAH修饰的PEEK支架可有效促进眼眶骨缺损的修复，OPN和Runx2的表达增加。负载BMP2的HAH有效增加了BMSCs在PEEK上的黏附、增殖和成骨分化。PEEK-BMP2-HAH支架有望成为眼眶骨折修复的新材料。
