Mohammed Abubakar A, Ozoje Michael O, De Campos John S, Ikeobi Christian O, Talabi Adewale O, Durosaro Samuel O, Ilori Babatunde M, Aderoju Johnson A
Department of Agricultural Education, Federal College of Education, (Special), Oyo, Oyo State, Nigeria.
Department of Animal Breeding and Genetics, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.
Trop Anim Health Prod. 2025 Mar 19;57(3):131. doi: 10.1007/s11250-025-04356-0.
Interferon regulatory factor 3 (IRF3) is one of the strongest positional candidate genes implicated in a host of health-related phenotypes such as general disease resistance. The study was carried out to genetically characterize IRF3 gene in, N'Dama, Muturu and White Fulani cattle. DNA was extracted from the blood samples using the Zymo-spin extraction kit. ARLEQUIN 2.0001 software was used to estimate the basic population genetic statistics while DnaSP version 5.10.01 was used to estimate genetic diversity indices and test for deviation from neutrality. A total of 18 and 29 Single nucleotide polymorphisms (SNPs) were observed using codon code aligner in exons 1-2 and 5-6 of the IRF3 gene respectively in the three cattle breeds after polymerase chain reaction and sequencing. In exons 1-2, the Muturu (MU) possessed the highest value of SNPs (16) and genetic diversity indices, while the N'Dama (ND) possessed the least (1). In exons 5-6, the highest value of SNPs (14) was observed in the White Fulani and the genetic diversity indices was also high while the Muturu had the least. Analysis of molecular variation (AMOVA) carried out for the loci under consideration revealed a higher level of variation among populations than within populations. It was therefore concluded that the IRF3 gene had many polymorphisms and was highly diversified in Nigerian cattle breeds.
干扰素调节因子3（IRF3）是与一系列健康相关表型（如一般抗病性）相关的最强候选基因之一。本研究旨在对恩达马牛、穆图鲁牛和白富拉尼牛的IRF3基因进行遗传特征分析。使用Zymo-spin提取试剂盒从血样中提取DNA。使用ARLEQUIN 2.0001软件估计基本群体遗传统计数据，而使用DnaSP 5.10.01版本估计遗传多样性指数并检验是否偏离中性。在三个牛品种中，经聚合酶链反应和测序后，分别在IRF3基因的外显子1-2和5-6中使用密码子编码比对器观察到总共18个和29个单核苷酸多态性（SNP）。在外显子1-2中，穆图鲁牛（MU）的SNP值（16）和遗传多样性指数最高，而恩达马牛（ND）最少（1）。在外显子5-6中，白富拉尼牛的SNP值最高（14），遗传多样性指数也很高，而穆图鲁牛最少。对所考虑的基因座进行的分子变异分析（AMOVA）显示，群体间的变异水平高于群体内。因此得出结论，IRF3基因具有许多多态性，并且在尼日利亚牛品种中高度多样化。
