Gümüş Alper, Zengi Oğuzhan, Kazezoğlu Cemal, Uçar Kamil Taha, Coşkun Cihan, Tek Semih, Doğan Nazife, Düz Muhammed Emin, Şener Gülsen, Köyüstü Kübra Nur
Department of Medical Biochemistry, University of Health Sciences, Cam and Sakura City Hospital, G-434 Street No: 2L, Başakşehir, İstanbul, PK:34480, Türkiye.
University of Health Sciences Turkey, Kanuni Sultan Süleyman Training and Research Hospital, Clinic of Biochemistry, Istanbul, Türkiye.
BMC Med Educ. 2025 Mar 19;25(1):398. doi: 10.1186/s12909-025-06981-7.
This survey study aims to evaluate knowledge of centrifuge operation and adherence to centrifugation protocols among laboratory personnel. These aspects are crucial in preventing pre-analytical errors. Additionally, the study seeks to assess the equipment infrastructure and staffing status within the participants' institutions.
The study involved 397 participants from various medical laboratories. A 16-item survey, using a 5-point Likert scale, was utilized to assess theoretical and practical knowledge of centrifuge operation. Two groups of subscales were developed, with one focusing on theoretical and practical knowledge and the other on pre-centrifugation, centrifugation, and post-centrifugation processes. Demographic data were collected, and the reliability of the survey was analyzed using Cronbach's alpha (0.94). Validity was assessed through factor analysis.
The majority of participants were female (63%) and worked as laboratory technicians (72%). Approximately 71% had never received formal centrifuge training, and significant differences were observed between theoretical and practical knowledge, particularly among laboratory technicians (P < 0.001). Education level and job title also influenced knowledge scores (P < 0.001).
The developed survey scale demonstrated high reliability and validity, indicating its potential as a valuable reference for future studies assessing centrifugation competency and usage in laboratory environments. These findings underline the need for improved training programs to reduce pre-analytical errors and enhance overall laboratory performance.
本调查研究旨在评估实验室人员对离心机操作的知识掌握情况以及对离心方案的遵守情况。这些方面对于预防分析前误差至关重要。此外，该研究还旨在评估参与者所在机构的设备基础设施和人员配备状况。
该研究涉及来自各个医学实验室的397名参与者。使用一个16项的调查问卷，采用5点李克特量表来评估离心机操作的理论和实践知识。开发了两组子量表，一组侧重于理论和实践知识，另一组侧重于离心前、离心过程和离心后过程。收集了人口统计学数据，并使用克朗巴哈系数（0.94）分析了调查问卷的信度。通过因子分析评估效度。
大多数参与者为女性（63%），担任实验室技术员（72%）。约71%的人从未接受过正式的离心机培训，理论知识和实践知识之间存在显著差异，尤其是在实验室技术员中（P < 0.001）。教育水平和职称也影响知识得分（P < 0.001）。
所开发的调查问卷量表显示出高信度和效度，表明其有潜力作为未来评估实验室环境中离心能力和使用情况的研究的宝贵参考。这些发现强调了改进培训计划以减少分析前误差并提高整体实验室性能的必要性。
