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从离子色谱中收集生物活性化合物馏分：不再是不可能完成的任务。

Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible.

作者信息

Yates Hans S A, Carter James F, Fletcher Mary T, Santiago Viviene S, Thompson Ondrea, Hungerford Natasha L

机构信息

Queensland Health Coronial and Public Health Sciences (QHCPHS) Health and Food Sciences Precinct, Coopers Plains, QLD, Australia.

Queensland Alliance for Agriculture and Food Innovation (QAAFI), The University of Queensland. Health and Food Sciences Precinct, Coopers Plains, QLD, Australia.

出版信息

MethodsX. 2025 Feb 21;14:103243. doi: 10.1016/j.mex.2025.103243. eCollection 2025 Jun.

DOI:10.1016/j.mex.2025.103243
PMID:40103767
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11919326/
Abstract

This paper demonstrates the fraction collection of the novel sugar trehalulose, using a modified ion chromatograph. The Ion Chromatography (IC) method, previously published for the analysis of trehalulose, was augmented with a suppressor and purpose-made switching valve unit. A sample of stingless bee honey was then run, following the three main steps:•Separation of trehalulose fraction•Lyophilization•Confirmation of trehalulose The method should be applicable to not only sugar analysis but to any bioactive compound separable by IC. The authors were not able to find a similar method within currently published literature.

摘要

本文展示了使用改良离子色谱仪对新型糖类海藻糖进行馏分收集的过程。先前发表的用于分析海藻糖的离子色谱（IC）方法，通过添加一个抑制器和特制切换阀装置得到了改进。随后对无刺蜂蜂蜜样本进行了分析，遵循以下三个主要步骤：

• 海藻糖馏分的分离

• 冻干

• 海藻糖的确认

该方法不仅应适用于糖类分析，还应适用于任何可通过离子色谱分离的生物活性化合物。作者在当前已发表的文献中未能找到类似方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/08f39c376bc6/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/6caef4668dc7/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/07da66de28fe/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/e471a14a544f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/40fc018d6bb2/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/9fd6bafbd790/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/81d00afe6f04/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/08f39c376bc6/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/6caef4668dc7/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/07da66de28fe/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/e471a14a544f/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/40fc018d6bb2/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/9fd6bafbd790/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/81d00afe6f04/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7685/11919326/08f39c376bc6/gr6.jpg

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本文引用的文献

1
Feeding Sugars to Stingless Bees: Identifying the Origin of Trehalulose-Rich Honey Composition.给无刺蜂喂食糖类：确定富含海藻糖蜂蜜成分的来源。
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2
Stingless bee honey, a novel source of trehalulose: a biologically active disaccharide with health benefits.无刺蜂蜂蜜，一种新型的海藻糖来源：具有健康益处的生物活性二糖。
Sci Rep. 2020 Jul 22;10(1):12128. doi: 10.1038/s41598-020-68940-0.
3
Oligosaccharides Isolated from MGO™ Manuka Honey Inhibit the Adhesion of , O157:H7 and to Human HT-29 cells.
从MGO™麦卢卡蜂蜜中分离出的低聚糖可抑制大肠杆菌O157:H7和福氏志贺菌对人HT-29细胞的黏附。
Foods. 2019 Oct 1;8(10):446. doi: 10.3390/foods8100446.