Imtiaz Rashed, Saha Protyaee, Saim Brishty Esfat M, Kamal Sadia, Shill Manik Chandra, Reza Hasan Mahmud, Jain Preeti
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh.
Oman Med J. 2024 Sep 30;39(5):e674. doi: 10.5001/omj.2024.105. eCollection 2024 Sep.
Antibiotic resistance in clinical isolates is often correlated to poor disease management or prolonged hospitalization. The study aimed to determine the prevalence of multidrug-resistant (MDR) isolates in clinical samples collected from Dhaka, Bangladesh.
We investigated 150 clinical samples for the presence of . The isolates were tested against 12 antibiotics categorizable into three groups based on action mechanism and nine classes based on chemical structure. The susceptibility patterns were analyzed using the Kirby-Bauer disk diffusion method on Mueller Hinton agar. The isolates were classified as MDR and extensively drug-resistant based on their resistance patterns against different antibiotics.
We used 100 isolates for this study. The highest prevalence of resistance was observed against penicillin G (96.0%), cefuroxime (75.0%), and clindamycin (71.0%). The isolates exhibited 33.0% and 43.0% resistance against imipenem and meropenem, respectively. Almost all (98.0%) isolates showed MDR. A quarter of the isolates (23.0%) were resistant to five classes of antibiotics and 18.0% to six classes of antibiotics. Moreover, 20.0% of isolates exhibited extensive drug resistance. Among the cell wall synthesis inhibitors, the highest resistance was observed against penicillin G (31.0%). Resistance rates were in the range 20-30% against protein synthesis inhibitors, clindamycin (29.0%), tetracycline (21.0%), and chloramphenicol (21.0%). Among the isolates, 90.0% showed multiple antibiotic resistance index scores of ≥ 0.3, while 65.0% scored ≥ 0.5. The carbapenem group accounted for the maximum sensitivity (62.0%), followed by 60.0% each for gentamicin and norfloxacin.
The high prevalence of MDR isolates in clinical samples from Bangladesh indicates a serious threat to public health. Our findings emphasize the urgent need to control MDR through promoting responsible use of suitable antibiotics and public awareness of the dangers of antibiotic misuse, increasing testing facilities, and promoting genetic research.
临床分离株中的抗生素耐药性通常与疾病管理不善或住院时间延长相关。本研究旨在确定从孟加拉国达卡收集的临床样本中多重耐药（MDR）分离株的流行情况。
我们调查了150份临床样本中是否存在……。对分离株针对12种抗生素进行测试，这些抗生素根据作用机制可分为三组，根据化学结构可分为九类。在穆勒-欣顿琼脂上使用 Kirby-Bauer 纸片扩散法分析药敏模式。根据分离株对不同抗生素的耐药模式将其分类为多重耐药和广泛耐药。
本研究使用了100株……分离株。观察到对青霉素G（96.0%）、头孢呋辛（75.0%）和克林霉素（71.0%）的耐药率最高。分离株对亚胺培南和美罗培南的耐药率分别为33.0%和43.0%。几乎所有（98.0%）分离株都表现出多重耐药。四分之一的分离株（23.0%）对五类抗生素耐药，18.0%对六类抗生素耐药。此外，20.0%的分离株表现出广泛耐药。在细胞壁合成抑制剂中，观察到对青霉素G的耐药率最高（31.0%）。对蛋白质合成抑制剂、克林霉素（29.0%）、四环素（21.0%）和氯霉素（21.0%）的耐药率在20%至30%之间。在……分离株中，90.0%的多重抗生素耐药指数得分≥0.3，而65.
