Li Cheng, Hu Pengnan, Fan Chenglong, Mi Hua
Department of Urology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Transl Cancer Res. 2025 Feb 28;14(2):1008-1023. doi: 10.21037/tcr-24-1509. Epub 2025 Feb 26.
Long non-coding RNA (lncRNA) small nucleolar RNA host gene 3 () has been reported to be involved in the pathological process of a variety of tumors, including clear cell renal cell carcinoma (ccRCC). However, whether can be used as a prognostic biomarker and its correlation with immune infiltration in ccRCC remain unclear, warranting further research. This study aims to explore the relationship between and immune infiltration in ccRCC and confirm the potential of to predict survival of ccRCC patients.
The Cancer Genome Atlas (TCGA) database was used to assess the expression of in ccRCC, evaluate clinicopathological characteristics, assess prognosis, and conduct functional enrichment analysis. The ccRCC microenvironment and immune infiltration were investigated using the Estimation of STromal and Immune cells in MAlignant Tumor tissues using Expression data (ESTIMATE) and Cell-type Identification By Estimating Relative Subsets Of RNA Transcripts (CIBERSORT) algorithms, respectively. We additionally investigated the relationships between and immunological checkpoints. Drug sensitivity of was investigated in R. The expression of was verified in the Gene Expression Omnibus (GEO) database, ccRCC cell lines, and tissues. Wound healing and Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assays were used to evaluate tumor cell migration and proliferation. Fluorescence in situ hybridization (FISH) assay was conducted to localize in ccRCC cells.
expression was significantly upregulated in ccRCC cells and tissues and associated with several clinicopathological features and poor prognosis of ccRCC patients. was correlated with immune cells infiltration in ccRCC and exhibited sensitivity to various targeted and chemotherapy drugs. Knockdown of significantly reduced the proliferation and migration of ccRCC. FISH results showed that was located in the cell nucleus.
Overall, this study demonstrates that is a prognostic biomarker correlated with immune infiltration in ccRCC.
长链非编码RNA（lncRNA）小核仁RNA宿主基因3（）已被报道参与包括透明细胞肾细胞癌（ccRCC）在内的多种肿瘤的病理过程。然而，在ccRCC中是否可作为预后生物标志物及其与免疫浸润的相关性仍不清楚，需要进一步研究。本研究旨在探讨在ccRCC中与免疫浸润的关系，并确认预测ccRCC患者生存的潜力。
使用癌症基因组图谱（TCGA）数据库评估在ccRCC中的表达，评估临床病理特征，评估预后，并进行功能富集分析。分别使用基于表达数据的恶性肿瘤组织中基质和免疫细胞估计（ESTIMATE）算法和通过估计RNA转录本相对子集进行细胞类型鉴定（CIBERSORT）算法研究ccRCC微环境和免疫浸润。我们还研究了与免疫检查点之间的关系。在R中研究了的药物敏感性。在基因表达综合数据库（GEO）、ccRCC细胞系和组织中验证了的表达。使用伤口愈合和甲基噻唑基二苯基四氮唑溴盐（MTT）试验评估肿瘤细胞迁移和增殖。进行荧光原位杂交（FISH）试验以在ccRCC细胞中定位。
在ccRCC细胞和组织中表达显著上调，并且与ccRCC患者的几种临床病理特征和不良预后相关。与ccRCC中的免疫细胞浸润相关，并且对各种靶向和化疗药物表现出敏感性。敲低显著降低了ccRCC的增殖和迁移。FISH结果显示位于细胞核中。
总体而言，本研究表明是与ccRCC中免疫浸润相关的预后生物标志物。
