Gao Xiao-Tian, Zhang Hai-Mo, Zhang Xiao-Zu, Wang Yi-Jing, Bi Hui-Ning, Yu Miao, Li Yan, Wang Xiao-Li
School of Medical Imaging, Shandong Second Medical University, Weifang, Shandong 261053, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2025 Mar 15;27(3):366-372. doi: 10.7499/j.issn.1008-8830.2411071.
To evaluate preterm white matter injury (PWMI) in neonatal rats using multimodal magnetic resonance imaging (MRI) combined with histological assessments and to explore its underlying mechanisms.
Healthy 3-day-old Sprague-Dawley neonatal rats were randomly divided into a sham operation group and a PWMI group (=12 in each group). A PWMI model was established in neonatal rats through hypoxia-ischemia. Laser speckle imaging was used to observe changes in cerebral oxygen saturation and blood flow at different time points post-modeling. Multimodal MRI was employed to assess the condition of white matter injury, while hematoxylin-eosin staining was utilized to observe morphological changes in the striatal area on the injured side. Immunofluorescence staining was performed to detect the proliferation and differentiation of oligodendrocyte precursor cells.
At 0, 6, 12, 24, and 72 hours post-modeling, the relative blood flow and relative oxygen saturation on the injured side in the PWMI group were significantly lower than those in the sham operation group (<0.05). At 24 hours post-modeling, T2-weighted imaging showed high signals in the white matter of the injured side in the PWMI group, with relative apparent diffusion coefficient values and Lorenz differential values being lower than those in the sham operation group (<0.001); additionally, the arrangement of nerve cells in the PWMI group was disordered, and the number of EdUPDGFR-α cells was higher than that in the sham operation group (<0.001). At 28 days post-modeling, the relative fractional anisotropy values, the number of EdUOlig2 cells, and the fluorescence intensity of myelin basic protein and neurofilament protein 200 in the white matter region of the PWMI group were all lower than those in the sham operation group (<0.001).
Multimodal MRI can evaluate early and long-term changes in PWMI in neonatal rat models , providing both imaging and pathological evidence for the diagnosis and treatment of PWMI in neonates. Hypoxia-ischemia inhibits the proliferation and differentiation of oligodendrocyte precursor cells in neonatal rats, leading to PWMI.
采用多模态磁共振成像（MRI）结合组织学评估来评估新生大鼠的早产白质损伤（PWMI），并探讨其潜在机制。
将健康的3日龄Sprague-Dawley新生大鼠随机分为假手术组和PWMI组（每组n = 12）。通过缺氧缺血在新生大鼠中建立PWMI模型。利用激光散斑成像观察建模后不同时间点脑氧饱和度和血流的变化。采用多模态MRI评估白质损伤情况，同时利用苏木精-伊红染色观察损伤侧纹状体区域的形态学变化。进行免疫荧光染色以检测少突胶质前体细胞的增殖和分化。
建模后0、6、12、24和72小时，PWMI组损伤侧的相对血流和相对氧饱和度显著低于假手术组（P < 0.05）。建模后24小时，T2加权成像显示PWMI组损伤侧白质呈高信号，相对表观扩散系数值和Lorenz差值低于假手术组（P < 0.001）；此外，PWMI组神经细胞排列紊乱，EdU+PDGFR-α细胞数量高于假手术组（P < 0.001）。建模后28天，PWMI组白质区域的相对各向异性分数值、EdU+Olig2细胞数量以及髓鞘碱性蛋白和神经丝蛋白200的荧光强度均低于假手术组（P < 0.001）。
多模态MRI可评估新生大鼠模型中PWMI的早期和长期变化，为新生儿PWMI的诊断和治疗提供影像学和病理学证据。缺氧缺血抑制新生大鼠少突胶质前体细胞的增殖和分化，导致PWMI。
