Mohammad Mahathir, Richi Fahmida Tasnim, Arafat Md, Jiko Pair Ahmed, Haque Mohammad Rashedul, Hossain Md Hemayet, Hossain Md Sakhaoyat, Ashrafi Sania, Taher Mohammad Abdullah, Alam Safaet
Department of Chemistry, Chittagong University of Engineering & Technology, Chittagong, Bangladesh.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, Dhaka, Bangladesh.
Chem Biodivers. 2025 Aug;22(8):e202500261. doi: 10.1002/cbdv.202500261. Epub 2025 Apr 12.
Cheilocostus speciosus (J. Koenig) C. Specht, commonly known as "crepe-ginger," is a traditional plant with edible flowers utilized in folk medicine. This study uses crepe-ginger flowers to assess their hepatoprotective effects through the management of oxidative stress. C. speciosus flower's methanolic extract (CSF-ME) was subjected to in vitro antioxidant effects were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and in vivo by catalase (CAT) assays which ameliorated CCl-induced hepatic injury evident by histopathological analysis. The chemical assay was evaluated via phytochemical screening and gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis followed by in silico studies. The antioxidant assay DPPH (IC = 179.36 µg/mL) and ABTS (IC = 198.27 µg/mL) showed remarkable scavenging activity. Hepatotoxicity experiments demonstrated that CSF-ME improved liver function by positively regulating AST, ALT, ALP, bilirubin, creatinine, LDL, CHO, TG, HDL, and catalase levels. Besides, histopathological analysis revealed normal hepatocyte integrity and microstructures after treatment. Besides, phytochemical screening revealed prospective phytochemical groups, while GC-MS/MS analysis recognized 40 compounds, resulting in auspicious outcomes employing computer-aided studies. The findings indicated that the CSF-ME possesses promising hepatoprotective and antioxidant prospects, which demand further extensive research to develop novel lead compounds from this natural source.
闭鞘姜（Cheilocostus speciosus (J. Koenig) C. Specht），俗称“绉叶姜”，是一种传统植物，其花朵可食用，在民间医学中有所应用。本研究使用绉叶姜花，通过管理氧化应激来评估其肝脏保护作用。对闭鞘姜花的甲醇提取物（CSF-ME）进行了体外抗氧化作用评估，使用2,2-二苯基-1-苦基肼（DPPH）和2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）进行测定，并通过过氧化氢酶（CAT）测定进行体内评估，通过组织病理学分析改善了四氯化碳诱导的肝损伤。通过植物化学筛选和气相色谱-串联质谱（GC-MS/MS）分析进行化学测定，随后进行计算机模拟研究。抗氧化测定DPPH（IC = 179.36 μg/mL）和ABTS（IC = 198.27 μg/mL）显示出显著的清除活性。肝毒性实验表明，CSF-ME通过正向调节AST、ALT、ALP、胆红素、肌酐、低密度脂蛋白、胆固醇、甘油三酯、高密度脂蛋白和过氧化氢酶水平改善肝功能。此外，组织病理学分析显示治疗后肝细胞完整性和微观结构正常。此外，植物化学筛选揭示了潜在的植物化学基团，而GC-MS/MS分析识别出40种化合物，通过计算机辅助研究取得了良好结果。研究结果表明，CSF-ME具有有前景的肝脏保护和抗氧化前景，需要进一步广泛研究以从这种天然来源开发新型先导化合物。
