Guo Enming, Chen Xiaolong, Wu Xuanxuan, Su Zerui, Lai Yong, Yang Chaochen, Li Mingwan, Zhang Dangquan
College of Forestry, Henan Agricultural University, Zhengzhou, Henan, China.
Chem Biodivers. 2025 Mar 31:e202402803. doi: 10.1002/cbdv.202402803.
Yulania biondii contains diverse bioactive compounds with significant application potential in the pharmaceutical, food, and cosmetics industries. This study investigates the bioactivity and composition of extracts from Y. biondii. It gives us a way to think about how to use forestry waste resources in a more complete way. Extracts from Y. biondii branches, leaves, and fruits were obtained using solvents such as water, ethanol, and acetone. The chemical components were analyzed using Fourier transform infrared spectroscopy (FT-IR), gas chromatography-mass spectrometry (GC-MS), and high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS). The antimicrobial activity of the extracts was assessed using the minimum inhibitory concentration (MIC) method, whereas antioxidant capacity was evaluated using DPPH and ABTS radical scavenging assays. Antitumor activity against HeLa, K562, SGC-7901, MCF-7, and HepG2 cells was assessed using the MTT assay. The results indicate that Y. biondii extracts contain a high content of flavonoids, alkaloids, phenolic compounds, and organic acids, with the ethanol extract showing the highest proportion of bioactive components at 64.93%. Additionally, Y. biondii extracts exhibited antioxidant, antibacterial, and antitumor properties, with particularly strong antioxidant effects that increased with higher extract concentrations. Among the extraction parts, the branch extract demonstrated the strongest antioxidant capacity, followed by the fruit extract, with the leaf extract showing the lowest capacity. Regarding antibacterial activity, the ethanol and acetone extracts from leaves and branches displayed strong inhibitory effects against Gram-positive bacteria. The ethanol extract from branches showed the strongest inhibition against Staphylococcus aureus, whereas the acetone extract from leaves exhibited the best inhibition against Bacillus cereus. Both ethanol and water extracts displayed varying levels of inhibition against five common tumor cell lines, with the antitumor activity ranked as follows, from strongest to weakest: leukemia cells, human gastric cancer cells, cervical cancer cells, human liver cancer cells, and human breast cancer cells. Notably, the water extract from Y. biondii leaves exhibited the strongest inhibition against human gastric cancer cells, with an inhibition rate of 82.49%. This study revealed the compositional characteristics of Y. biondii extracts and preliminarily evaluated their potential in antibacterial, antioxidant, and antitumor applications, providing a theoretical reference for the further development and application of natural plant extracts.
望春玉兰含有多种生物活性化合物，在制药、食品和化妆品行业具有重要的应用潜力。本研究调查了望春玉兰提取物的生物活性和成分。它为我们提供了一种思考如何更全面地利用林业废弃物资源的方法。采用水、乙醇和丙酮等溶剂获得望春玉兰树枝、树叶和果实的提取物。使用傅里叶变换红外光谱（FT-IR）、气相色谱-质谱联用（GC-MS）和高效液相色谱-四极杆飞行时间质谱（HPLC-QTOF-MS）分析化学成分。采用最低抑菌浓度（MIC）法评估提取物的抗菌活性，而抗氧化能力则通过DPPH和ABTS自由基清除试验进行评估。使用MTT试验评估对HeLa、K562、SGC-7901、MCF-7和HepG2细胞的抗肿瘤活性。结果表明，望春玉兰提取物含有高含量的黄酮类、生物碱、酚类化合物和有机酸，乙醇提取物中生物活性成分的比例最高，为64.93%。此外，望春玉兰提取物具有抗氧化、抗菌和抗肿瘤特性，并具有特别强的抗氧化作用，且随着提取物浓度的升高而增强。在提取部位中，树枝提取物的抗氧化能力最强，其次是果实提取物，树叶提取物的能力最低。关于抗菌活性，树叶和树枝的乙醇和丙酮提取物对革兰氏阳性菌显示出较强的抑制作用。树枝的乙醇提取物对金黄色葡萄球菌的抑制作用最强，而树叶的丙酮提取物对蜡样芽孢杆菌的抑制效果最佳。乙醇提取物和水提取物对五种常见肿瘤细胞系均表现出不同程度的抑制作用，抗肿瘤活性从强到弱依次为：白血病细胞、人胃癌细胞、子宫颈癌细胞、人肝癌细胞和人乳腺癌细胞。值得注意的是，望春玉兰树叶的水提取物对人胃癌细胞的抑制作用最强，抑制率为82.49%。本研究揭示了望春玉兰提取物的成分特征，并初步评估了其在抗菌、抗氧化和抗肿瘤应用中的潜力，为天然植物提取物的进一步开发和应用提供了理论参考。
