Haghighat Khadijeh, Mahmoudi Fariba, Khoshkam Maryam, Khazali Homayoun
Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran.
Department of Chemistry, University of Mohaghegh Ardabili, Ardabil, Iran.
Korean J Pain. 2025 Apr 1;38(2):128-137. doi: 10.3344/kjp.24355. Epub 2025 Mar 20.
Chrysin is a natural flavonoid that exhibits various pharmacological activities including pain relief. However, the effects of chrysin on changes of metabolic profiles during pain remain unclear. The aim of this study was to analyze the biomarkers related to pain in serum and to evaluate the analgesic properties of chrysin in a rat model of pain.
Male Wister rats were divided into four groups (n = 5). Pain was induced by injecting 50 μL of formalin into the hind paw. Chrysin and diclofenac (10 mg/kg, intraperitoneal injection) was administered to the intact and pain groups. All injections were given 30 minutes before pain induction. Immediately, the behavioral test was performed. Then the serum sample was separated for HNMR-based metabolite analysis.
Chrysin treatment alleviated the paw licking events, flinching response, and pain score. The integrated analyses further revealed three major metabolic changes including glycine-serine-threonine, taurine-hypotaurine, and arginine by comparing the serums from intact operated rats, pain rats, and pain rats treated with chrysin, and suggested that chrysin may improve pain by regulating the biosynthesis of these metabolic pathways.
These findings provide insights into metabolic pathways involved in pain and the analgesic effects of chrysin and may help to identify potential targets for the anti-pain properties of chrysin.
白杨素是一种天然黄酮类化合物，具有多种药理活性，包括缓解疼痛。然而，白杨素对疼痛期间代谢谱变化的影响尚不清楚。本研究的目的是分析血清中与疼痛相关的生物标志物，并评估白杨素在大鼠疼痛模型中的镇痛特性。
将雄性Wister大鼠分为四组（n = 5）。通过向大鼠后爪注射50 μL福尔马林诱导疼痛。向完整组和疼痛组大鼠腹腔注射白杨素和双氯芬酸（10 mg/kg）。所有注射均在诱导疼痛前30分钟进行。随后立即进行行为测试。然后分离血清样本用于基于核磁共振氢谱的代谢物分析。
白杨素治疗减轻了舔爪次数、退缩反应和疼痛评分。通过比较完整手术大鼠、疼痛大鼠和接受白杨素治疗的疼痛大鼠的血清，综合分析进一步揭示了三个主要的代谢变化，包括甘氨酸-丝氨酸-苏氨酸、牛磺酸-亚牛磺酸和精氨酸，并表明白杨素可能通过调节这些代谢途径的生物合成来改善疼痛。
这些发现为疼痛相关的代谢途径以及白杨素的镇痛作用提供了见解，并可能有助于确定白杨素抗疼痛特性的潜在靶点。
