Wirdnam P K, Milner R D
Nephron. 1985;40(3):362-7. doi: 10.1159/000183494.
Rat Tamm-Horsfall glycoprotein (THG) was isolated from urine and disaggregated by gel filtration. Purity was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and autoradiography. Antibodies were raised and used in the development of a specific radioimmunoassay for rat THG. Parallelism could be demonstrated between the standard preparation and urine (diluted to 1:320 with distilled water) and serum (at all dilutions) samples. Recovery of standard added to urine or serum was on average 93% and inter- and intra-assay variation were both below 7%. There was a positive correlation between the mean urinary excretory rate of THG (microgram/24h) and body weight in female rats (p less than 0.001) but not in male rats. Serum THG levels were similar in both sexes and showed a positive correlation with body weight (p less than 0.001; range 43-240 ng/ml). It is concluded that the rat urinary THG excretion rate is similar to that in man and that rat serum also contains a THG-like protein.
从大鼠尿液中分离出Tamm-Horsfall糖蛋白（THG），并通过凝胶过滤使其解聚。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和放射自显影确认其纯度。制备抗体并用于开发大鼠THG的特异性放射免疫测定法。标准制剂与尿液（用蒸馏水稀释至1:320）和血清（所有稀释度）样本之间可证明具有平行性。添加到尿液或血清中的标准品回收率平均为93%，批间和批内变异均低于7%。雌性大鼠THG的平均尿排泄率（微克/24小时）与体重呈正相关（p<0.0），而雄性大鼠则无此相关性。两性血清THG水平相似，且与体重呈正相关（p<0.001；范围为43-240纳克/毫升）。结论是，大鼠尿THG排泄率与人相似，大鼠血清中也含有一种THG样蛋白。
