脂肪酸代谢相关基因MECR促进前列腺癌进展。

Fatty acid metabolism related gene MECR contributes to the progression of prostate cancer.

作者信息

Liu Yifan, Wan Lilin, Chen Yuxuan, Zhang Ruixin, Xia Yi, Chen Ming, Huang Xiang, Liu Ruiji

机构信息

Department of Urology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610072, China.

Southeast University, 87 Dingjia Bridge Hunan Road, Nanjing, China.

出版信息

Cancer Cell Int. 2025 Mar 19;25(1):105. doi: 10.1186/s12935-025-03738-6.

DOI:10.1186/s12935-025-03738-6

PMID:40108606

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11924791/

Abstract

BACKGROUND

Prostate cancer (PCa) is the most common urological malignancy and second only to lung cancer in incidence among men. Its prognosis varies widely due to its heterogeneity. Research indicates that fatty acid metabolism may play a role in tumor development.

METHODS

The gene expression profiles of PCa cell lines (GSE6919) in GEO database were analyzed to identify differentially expressed genes and their significance in relation to progression-free interval. The R package was employed to assess overall survival significance and clinicopathological features. The study investigated the effects of gene mutations and methylation on PCa and their correlation with immune cell infiltration in the tumor microenvironment, utilizing cBioPortal and UALCAN resources. TIMER was used in the TCGA project to compare the expression of MECR in tumours and in adjacent normal tissue for different tumours or for specific tumour subtypes. Furthermore, we examined the impact of hub genes on PCa progression through RT qPCR, immunohistochemistry, and cellular assays.

RESULTS

The MECR gene, which plays a role in fatty acid metabolism, has been implicated in the development and progression of PCa. Its expression levels are significantly associated with clinical features, survival outcomes, and prognosis in PCa. Comprehensive analyses of MECR mutations and methylation levels further underscore its involvement in the progression of prostate cancer. Additionally, MECR is closely associated with the immune microenvironment and immune cell infiltration in PCa. Furthermore, the in vitro and in vivo data indicated that MECR plays a role in PCa proliferation, migration, and invasion.

CONCLUSION

MECR has significant potential for research and application in the assessment of PCa prognosis and the regulation of the immune microenvironment.

摘要

背景

前列腺癌（PCa）是最常见的泌尿系统恶性肿瘤，在男性发病率中仅次于肺癌。由于其异质性，其预后差异很大。研究表明，脂肪酸代谢可能在肿瘤发展中起作用。

方法

分析基因表达综合数据库（GEO）中PCa细胞系（GSE6919）的基因表达谱，以鉴定差异表达基因及其与无进展生存期的关系。使用R软件包评估总生存意义和临床病理特征。利用cBioPortal和UALCAN资源，研究基因突变和甲基化对PCa的影响及其与肿瘤微环境中免疫细胞浸润的相关性。在癌症基因组图谱（TCGA）项目中使用肿瘤免疫估计资源（TIMER）比较不同肿瘤或特定肿瘤亚型中肿瘤组织和相邻正常组织中MECR的表达。此外，我们通过逆转录定量聚合酶链反应（RT qPCR）、免疫组织化学和细胞试验研究了关键基因对PCa进展的影响。

结果

在脂肪酸代谢中起作用的MECR基因与PCa的发生和发展有关。其表达水平与PCa的临床特征、生存结果和预后显著相关。对MECR突变和甲基化水平的综合分析进一步强调了其参与前列腺癌的进展。此外，MECR与PCa中的免疫微环境和免疫细胞浸润密切相关。此外，体外和体内数据表明，MECR在PCa增殖、迁移和侵袭中起作用。