Choi Sung Hoon, Lee Ha Young, Yun Sung Ho, Jang Sung Jae, Kim Seung Up, Park Jun Yong, Ahn Sang Hoon, Kim Do Young
Institute of Health & Environment, Seoul National University, Seoul, Korea.
KoBioLabs Inc., Seoul, Korea.
J Liver Cancer. 2025 Mar;25(1):123-133. doi: 10.17998/jlc.2025.03.08. Epub 2025 Mar 20.
BACKGROUNDS/AIMS: In hepatocellular carcinoma (HCC), which exhibits high mortality and recurrence rates globally, the traits of cancer stem cells (CSCs) that significantly influence recurrence and metastasis are not well understood. CSCs are self-renewing cell types identified in most liquid and solid cancers, contributing to tumor initiation, growth, resistance, recurrence, and metastasis following chemo-radiotherapy or trans-arterial chemoembolization therapy.
CSCs are classified based on the expression of cell surface markers such as CD133, which varies depending on the tumor type. Proteomic analysis of liver cancer cell lines with cancer stem cell potential and HCC cancer cell lines lacking stem cell propensity was conducted to compare and analyze specific expression patterns.
Proteomic profiling and enrichment analysis revealed higher expression of the calcium-binding protein S100 family in CD133+ Huh7 cells than in CD133- or wild-type cells. Furthermore, elevated expression of S100 family members was confirmed in an actual CD133+ liver cancer cell line via protein-protein network analysis and quantitative polymerase chain reaction (qPCR).
The S100 family members are not only new markers of cancer stem cells but will also assist in identifying new treatment strategies for CSC metastasis and tumor advancement.
背景/目的：在全球范围内具有高死亡率和复发率的肝细胞癌（HCC）中，对显著影响复发和转移的癌症干细胞（CSC）特性的了解尚不完善。CSC是在大多数液体和实体癌中发现的自我更新细胞类型，在化疗、放疗或经动脉化疗栓塞治疗后，会导致肿瘤起始、生长、耐药、复发和转移。
CSC根据细胞表面标志物如CD133的表达进行分类，其表达因肿瘤类型而异。对具有癌症干细胞潜能的肝癌细胞系和缺乏干细胞倾向的HCC癌细胞系进行蛋白质组分析，以比较和分析特定的表达模式。
蛋白质组分析和富集分析显示，与CD133-或野生型细胞相比，CD133+Huh7细胞中钙结合蛋白S100家族的表达更高。此外，通过蛋白质-蛋白质网络分析和定量聚合酶链反应（qPCR），在实际的CD133+肝癌细胞系中证实了S100家族成员的表达升高。
S100家族成员不仅是癌症干细胞的新标志物，还将有助于确定针对CSC转移和肿瘤进展的新治疗策略。
