Allele and genotype frequencies of variants in P450 cytochromes, transports, and DNA repair enzymes in the Dominican Republic population.

INTRODUCTION

Single-nucleotide variants (SNVs) give rise to important inter-individual and inter-ethnic variabilities in the metabolism and disposition of several therapeutic agents and may cause differences in the treatment response to clinically important drugs like antiarrhythmics, antidepressants, antihistamines, and antipsychotics, among others. Information about the prevalence of variants in the Dominican Republic population is still limited. The aim of this study was to describe the frequency distribution of 32 SNVs from 14 genes with pharmacogenetic interest within a sample of 150 unrelated healthy individuals.

METHODS

Genotype and allele frequencies were determined, and pairwise Wright's F statistic was evaluated.

RESULTS

Hardy-Weinberg equilibrium deviations were found in seven loci from (rs16947, rs3892097, rs1058164, rs1135840, and rs28371725) and (rs12769205 and rs4244285) genes. The minor allele frequencies ranged from 0.01 to 0.50 values in the xenobiotic biotransformation enzymes and transporter genes. The average admixture estimates were 51.6%, 39.5%, and 8.9% for European, African, and Amerindian ancestries, respectively. Pairwise F analysis revealed that Dominicans displayed genetic similarity to Latin American populations, especially those with Afro-Caribbean ancestry, given the selected variants. Higher differences were identified from East and South Asians, Europeans, and Africans, in which several values above the F threshold for moderate differentiation were identified within variants in , and genes.

CONCLUSIONS

These results should allow establishing the clinical relevance of pharmacogenetic testing in variant alleles related to drug transport and metabolism genes in this population.