棕榈果柄多糖的提取、分析及抗氧化活性
Extraction, analysis and antioxidant activity of palm peduncle polysaccharide.
作者信息
Zhou Shiyang, Wang Wenbin, Tan Jun
机构信息
Chongqing Chemical Industry Vocational College, Chongqing, 401228 China.
Chongqing Academy of Traditional Chinese Medicine, Chongqing, 400065 China.
出版信息
Food Sci Biotechnol. 2024 Dec 5;34(6):1479-1493. doi: 10.1007/s10068-024-01762-2. eCollection 2025 Apr.
The structural and antioxidant activities of palm peduncle polysaccharide was characterized, and its protect effect on hydrogen peroxide induced oxidative stress damaged for RAW264.7 cells was evaluated. The result of structural analysis showed that palm peduncle polysaccharide was main composed of rhamnoose, arabose, galactose and glucose, which accounted for 10.87%, 21.35%, 18.33% and 18.07%, respectively. The result of antioxidant activities in vitro showed that palm peduncle polysaccharide could significant increased contents of CAT, SOD, GSH-Px in superneant for RAW264.7 cells induced by HO, while the contents of MDA was decreased, showing a dose-effect relationship. In vivo activity test, palm peduncle polysaccharide could significant increased of SOD, GSH-Px activity in serum and liver of mice, and significant reduced of MDA content. The result showed that palm peduncle polysaccharide could significant increased the contents or gene expression of Nrf2, HO-1, NQO1 proteins relate to oxidative stress pathway in RAW264.7 cells damaged by HO.
对棕榈果柄多糖的结构和抗氧化活性进行了表征，并评估了其对过氧化氢诱导的RAW264.7细胞氧化应激损伤的保护作用。结构分析结果表明，棕榈果柄多糖主要由鼠李糖、阿拉伯糖、半乳糖和葡萄糖组成，其含量分别为10.87%、21.35%、18.33%和18.07%。体外抗氧化活性结果表明，棕榈果柄多糖可显著提高HO诱导的RAW264.7细胞上清液中CAT、SOD、GSH-Px的含量，同时降低MDA含量，呈现剂量效应关系。体内活性试验表明，棕榈果柄多糖可显著提高小鼠血清和肝脏中SOD、GSH-Px的活性，并显著降低MDA含量。结果表明，棕榈果柄多糖可显著提高HO损伤的RAW264.7细胞中与氧化应激途径相关的Nrf2、HO-1、NQO1蛋白的含量或基因表达。
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