College of Food Science & Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China.
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China.
Carbohydr Polym. 2018 Jun 15;190:67-76. doi: 10.1016/j.carbpol.2017.11.090. Epub 2017 Nov 26.
The in vitro activities of 39 polysaccharides from different parts of 13 lotus root varieties were evaluated and introduced into multiple linear regression analysis to explore the structure-activity relationships using their chromatographic fingerprint features as independent variables. Their 2,2-diphenyl-1-picrylhydrazyl/hydroxyl radical scavenging abilities, ferric reducing antioxidant powers and growth-inhibitory effects against HepG2 and SGC7901 cancer cells were all diverse, with the variable-coefficients ranging from 24.49% to 87.76%, while their macrophage immunostimulatory activities evaluated by nitric oxide production and tumor necrosis factor-alpha secretion showed relatively low variations. Lotus root polysaccharides (LRPs) from the peels and nodes possessed stronger activities than those from the fleshes. Their fingerprint-activity relationship models indicated that monosaccharide composition was closely related to the activities, but not molecular weight. LRPs have health-improving potentials, and their activities can be partly predicted by the quantitative fingerprint-activity relationship model.
评估了 13 个莲藕品种不同部位的 39 种多糖的体外活性，并将其引入多元线性回归分析，用其色谱指纹特征作为自变量，探讨结构-活性关系。它们的 2,2-二苯基-1-苦基肼/羟基自由基清除能力、铁还原抗氧化能力以及对 HepG2 和 SGC7901 癌细胞的生长抑制作用各不相同，变异系数范围为 24.49%至 87.76%，而通过一氧化氮产生和肿瘤坏死因子-α分泌评估的巨噬细胞免疫刺激活性变化相对较小。来自莲藕皮和节点的莲藕多糖（LRP）比来自肉质部分的具有更强的活性。它们的指纹-活性关系模型表明，单糖组成与活性密切相关，但与分子量无关。LRP 具有改善健康的潜力，其活性可以通过定量指纹-活性关系模型进行部分预测。
