Muchová Monika, Münster Lukáš, Valášková Kristýna, Lovecká Lenka, Víchová Zdenka, Osička Josef, Kašpárková Věra, Humpolíček Petr, Vašíček Ondřej, Vícha Jan
Centre of Polymer Systems, Tomas Bata University in Zlín, tř. Tomáše Bati 5678, 760 01 Zlín, Czech Republic.
Centre of Polymer Systems, Tomas Bata University in Zlín, tř. Tomáše Bati 5678, 760 01 Zlín, Czech Republic; Department of Fat, Surfactant and Cosmetics Technology, Faculty of Technology, Tomas Bata University in Zlín, nám. T. G. Masaryka 5555, 760 01 Zlín, Czech Republic.
Int J Biol Macromol. 2025 May;308(Pt 1):142105. doi: 10.1016/j.ijbiomac.2025.142105. Epub 2025 Mar 18.
The study introduces a novel method for fabricating crosslinked chitosan/polypyrrole (PPy) composite nanofibers with covalently anchored PPy. Crosslinking is achieved already during electrospinning by using dialdehyde cellulose (DAC) as a dual-functioning reagent able to simultaneously crosslink chitosan nanofibers and covalently tether PPy nanoparticles by a newly discovered aldol condensation reaction. The presented method eliminates the need for postprocessing steps. It reduces the environmental impact by avoiding toxic organic chemicals while preventing PPy leaching and improving prepared composite nanofibers' mechanical and biological properties. A direct comparison to neat chitosan nanofibres was performed to demonstrate the superiority of prepared composites. The resulting crosslinked CHIT_DAC_PPy composite nanofibers have increased tensile strength, improved stability at low pH, conductivity up to 11 mS/cm, and higher swelling compared to neat CHIT nanofibers. They also possess significantly enhanced antibacterial activity against gram-positive S. aureus, higher antioxidant activity, increased immunomodulatory effects, and substantially higher acceleration of wound healing in vitro. CHIT_DAC_PPy nanofibrous composite thus shows significant potential for fabricating advanced wound dressings.
该研究介绍了一种制备具有共价锚定聚吡咯（PPy）的交联壳聚糖/聚吡咯复合纳米纤维的新方法。通过使用二醛纤维素（DAC）作为双功能试剂，在静电纺丝过程中即可实现交联，该试剂能够通过新发现的醛醇缩合反应同时交联壳聚糖纳米纤维并共价连接PPy纳米颗粒。所提出的方法无需后处理步骤。它通过避免使用有毒有机化学品减少了对环境的影响，同时防止PPy浸出并改善了制备的复合纳米纤维的机械和生物学性能。与纯壳聚糖纳米纤维进行了直接比较，以证明所制备复合材料的优越性。与纯CHIT纳米纤维相比，所得的交联CHIT_DAC_PPy复合纳米纤维具有更高的拉伸强度、在低pH值下更好的稳定性、高达11 mS/cm的电导率以及更高的溶胀性。它们还对革兰氏阳性金黄色葡萄球菌具有显著增强的抗菌活性、更高的抗氧化活性、增强的免疫调节作用以及在体外显著更高的伤口愈合促进作用。因此，CHIT_DAC_PPy纳米纤维复合材料在制造先进伤口敷料方面显示出巨大潜力。
